To filter or not to filter, that is the question

[penewbie]

Every "purity" test you see of UGL peptides is conducted AFTER being filtered with a .22um syringe filter. Contaminants larger than that, which are common, are removed first.

Unless you're filtering first, you're not injecting what you see in the test result, but the "raw" version with some unknown amount of contamination, consisting of glass delimitation shards, rubber particulates, bacteria, peptide aggregates, etc.

Whoa, that's crazy, I had no idea.

 

[GLiPtest]

Whoa, that's crazy, I had no idea.

It doesn't seem to be a commonly understood limitation of the type of testing we have access to.

The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for.

The inorganic particulates in cheap Chinese vials not certified to meet USP (the standards setting organization the FDA follows) particulate standards alone warrants filtering.

Injected glass and rubber particulates have been found lodged in many organs during autopsies. They create "micro embolisms" and/or chronic inflammation. With no natural means for glass or rubber to be broken down or removed, they accumulate with each injection.

 

[penewbie]

It doesn't seem to be a commonly understood limitation of the type of testing we have access to.

The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for.

The physical particulates in cheap Chinese vials not certified to meet USP (the standards setting organization the FDA follows) particulate standards alone warrants filtering.

Injected glass and rubber particulates have been found lodged in many organs during autopsies. They create "micro embolisms" and/or chronic inflammation. With no natural means for glass or rubber to be broken down or removed, they accumulate with each injection.

View attachment 6200

To be fair I always filter.

I do it because I listened to Ghoul on meso, who is a total fear mongering loudmouth, but he does make good points every now and again.

I'm not worried about bacteria in subq shots, but he showed that immunogenicity could be a realistic concern, so I filter.

It's a "nice" surprise to now be able to add another reason to my filtering, strengthening my decision.

 

[Garbo]

It doesn't seem to be a commonly understood limitation of the type of testing we have access to.

The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for.

The physical particulates in cheap Chinese vials not certified to meet USP (the standards setting organization the FDA follows) particulate standards alone warrants filtering.

Injected glass and rubber particulates have been found lodged in many organs during autopsies. They create "micro embolisms" and/or chronic inflammation. With no natural means for glass or rubber to be broken down or removed, they accumulate with each injection.

View attachment 6200

Hi and welcome, brand new member.

I can appreciate that "The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for."

But are you stating here that JANOSHIK TESTS of GLPs etc are "conducted AFTER being filtered with a .22um syringe filter"?

... which would mean that every time we confirm our product purchases with a Jano test, we are not actually learning what's in our own (unfiltered) vials?
And that we are not actually using what we think we are UNLESS we filter it that way?

Because I have never, ever come across that piece of information.

Nor do I filter.

 

[Vicki]

Hi and welcome, brand new member.

I can appreciate that "The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for."

But are you stating here that JANOSHIK TESTS of GLPs etc are "conducted AFTER being filtered with a .22um syringe filter"?

... which would mean that every time we confirm our product purchases with a Jano test, we are not actually learning what's in our own (unfiltered) vials?
And that we are not actually using what we think we are UNLESS we filter it that way?

Because I have never, ever come across that piece of information.

Nor do I filter.

All the testing companies have to do this to protect their equipment. Water soluble stuff does not get trapped in the filter so theres no loss of the peptides.

Also edited to add: they don't test for what's in the vial. They only test for what you specifically ask for. Tirz test will only test for tirz quantity and mass. It won't tell you if there's anything else in the vial.

 

[Mr. Smith]

Yes, all labs filter before testing... So, your Vial may contain, who knows what.. I always filter.

 

[Garbo]

View attachment 6203

Yes, all labs filter before testing... So, your Vial may contain, who knows what.. I always filter.

THANK YOU for that screenshot! I just wanted confirmation that JANO did it.

Appreciate it, and am astounded you were able to grab it so fast ... wait a minute — are you the Mr. Smith from The Matrix by any chance?

 

[Garbo]

All the testing companies have to do this to protect their equipment. Water soluble stuff does not get trapped in the filter so theres no loss of the peptides.

Also edited to add: they don't test for what's in the vial. They only test for what you specifically ask for. Tirz test will only test for tirz quantity and mass. It won't tell you if there's anything else in the vial.

Thanks, Vicki!

(I had known your second paragraph stuff, but just wanted to confirm Janoshik was counted among "ALL" the testing companies doing the first paragraph stuff, lol)

 

[Mr. Smith]

THANK YOU for that screenshot! I just wanted confirmation that JANO did it.

Appreciate it, and am astounded you were able to grab it so fast ... wait a minute — are you the Mr. Smith from The Matrix by any chance?

No, but you might know me from my infatuation with Ning Yang Bing on QUAL.

I took that screenshot from SWISS under lab chat with Jano and TP.

 

[Garbo]

No, but you might know me from my infatuation with Ning Yang Bing on QUAL.

I took that screenshot from SWISS under lab chat with Jano and TP.

hahah I would tell you hands off Ning, mister, but now I'm kinda infatuated with you.

 

[Garbo]

It doesn't seem to be a commonly understood limitation of the type of testing we have access to.

The standard in science, pharma, and regulatory agencies requires multiple methods of testing peptide drugs for contamination since they all have "blind spots" that need to be compensated for.

The physical particulates in cheap Chinese vials not certified to meet USP (the standards setting organization the FDA follows) particulate standards alone warrants filtering.

Injected glass and rubber particulates have been found lodged in many organs during autopsies. They create "micro embolisms" and/or chronic inflammation. With no natural means for glass or rubber to be broken down or removed, they accumulate with each injection.

Hey, Glip,

This is my second reply to this post.

In my first, I was stunned and questioning.

But many folks here have set me straight!

So I want to offer you a mea culpa AND thank you so much for helping make me smarter than I was yesterday

 

[nonyabizznez]

No, but you might know me from my infatuation with Ning Yang Bing on QUAL.

Ning sent me a PM and wanted me to pass this on.

She's busy trying to make a beige day pink!

hahah I would tell you hands off Ning, mister, but now I'm kinda infatuated with you.

This won't surprise her she says @Mr Smith has that effect on people, besides her mom was a Ho.

 

[nonyabizznez]

Hey, Glip,

This is my second reply to this post.

In my first, I was stunned and questioning.

But many folks here have set me straight!

So I want to offer you a mea culpa AND thank you so much for helping make me smarter than I was yesterday

I had no idea testing facilities filtered.

I'm especially happy I found a source with Ks-tek (packaged) filters on AliExpress under $90 shipped for 100! Arrived in less than 2wks!

I just found this on AliExpress:
$12.99 | Sterile hydrophilic Syringe Filters,PES Membrane 0.22μm Pore Size,4mm Diameter,25pcs/100 Pcs Individually Packed by Ks-Tek

https://a.aliexpress.com/_msElNGF

So @exploitedworkerbee there's an alternative to Peptide Test.

 

[Mr. Smith]

I had no idea testing facilities filtered.

I'm especially happy I found a source with Ks-tek (packaged) filters on AliExpress under $90 shipped for 100! Arrived in less than 2wks!

I just found this on AliExpress:
$12.99 | Sterile hydrophilic Syringe Filters,PES Membrane 0.22μm Pore Size,4mm Diameter,25pcs/100 Pcs Individually Packed by Ks-Tek

https://a.aliexpress.com/_msElNGF

So @exploitedworkerbee there's an alternative to Peptide Test.

Here is some more detailed info I got from TP.

 

[GLiPtest]

Here is some more detailed info I got from TP.

View attachment 6204

That's interesting. It means compared to labs using .22um filters, the same sample results from TP could be expected to show a lower purity.

 

[penewbie]

That's interesting. It means compared to labs using .22um filters, the same sample results from TP could be expected to show a lower purity.

Not really though.

That size difference is meaningless when it comes to small molecules like peptides

 

[GimmeABreak]

Does anyone know if PT and Jano filter for sterility testing? Seems like it could give you a false negative.

 

[GLiPtest]

Not really though.

That size difference is meaningless when it comes to small molecules like peptides

It has nothing to do with the size of the peptide molecule.

It's about the microorganisms and other contaminants that would otherwise be removed at .22um that pass through a .45um filter. This additional material reduces purity calculations.

 

[GLiPtest]

Does anyone know if PT and Jano filter for sterility testing? Seems like it could give you a false negative.

No, the sterility testing protocol doesn't involve filtration.

 

[penewbie]

It has nothing to do with the size of the peptide molecule.

It's about the microorganisms and other contaminants that would otherwise be removed at .22um that pass through a .45um filter. This additional material reduces purity calculations.

Microorganisms and contaminants do not enter into the purity calculations.