Do You Filter Your Peptides Before Use?

Jfrick11 said:
When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?
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You would want to put it into a new vial or you are wasting your time and energy.
 
Peptelligence said:
Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:
  • Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
  • Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.
The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:
  • Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
  • Maximizing the purity of your product, which directly translates to more reliable outcomes
  • Protecting yourself from potential contaminants introduced during manufacturing or shipping
Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:
  • Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
  • Make sure you have sterile syringes to use with the filters
  • Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)
Simple Process:
  1. Draw your reconstituted peptide into a sterile syringe
  2. Attach the filter to the syringe
  3. Slowly push the liquid through the filter into your sterile storage vial
  4. Label appropriately with contents and date
Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!
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I don't filter my peptides. Janoshik wrote: "Given what I've seen around during all the years, infections from subcutaneous injections are... extremely rare even with the worst of practices."
 
flsailfish22 said:
I don't filter my reconstituted peptides, but I will add some extra BA sometimes. The BA is what's going to safeguard you more than filtering from bacteria. I do filter the stuff I homebrew. Filtering is really more for particles/materials than could have fallen into the solution and BA it to make sure there is no bacteria. Filtering does add an extra layer of safety which isn't a bad thing. The type and size of filter listed is spot on the best way to go if you are going to start filtering your peptides.

As for refrigerating BAC. Hospira recommends against refrigeration even when opened. I however do refrigerate after opening. Hospitals don't refrigerate it either, but like I said above sometimes I will add a little more BA to make it like 1% instead of .9% for a little extra safety. Also, BAC is cheap. Don't use shit Amazon BAC. Buy Hospira BAC. I personally have like 20 3ml bottles at all times. If I really doubt something I just throw it away to lower risk.
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What olis BA? And how much do you add to a vial?
 
As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.

I’ve never stored a vial for more than 5 days though.
 
AndyPanda said:
As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.
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You're definitely injecting yourself with micro shards of glass.
 
zpped said:
No maybe about it. There is tons of literature on it. You're not going to die but the effects can be cumulative when you start getting weird muscle pain 10 years from now.
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I have shrapnel in my leg. It can’t be worse than that.
 
AndyPanda said:
As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.

I’ve never stored a vial for more than 5 days though.
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While I appreciate the credit, I was not the one who told you about those!
 
Skidude said:
I don't use ampules, and the conflicting opinions on the need/efficacy of filtering have not got me off the fence. I am not familiar with blunt tip needles, is that like blinker fluid?
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And left handed screwdrivers! I THINK they filter on the way into the syringe from the ampule, then you just switch the hypodermic a inject a cleaner fluid.
 
Skidude said:
I don't use ampules, and the conflicting opinions on the need/efficacy of filtering have not got me off the fence. I am not familiar with blunt tip needles, is that like blinker fluid?
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filter needles are not the same as syringe filters.
Filter needles are mandatory when using glass ampules.


AndyPanda said:
You’re smart. How do they work?
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There is a filter built into the needle.
You break the ampule (using good technique and a cotton ball to catch a lot of the shards)
You draw the fluid using the filter needle.
You throw away the filter needle and then put on whatever needle you want to inject it with (into yourself or into a vial of tirz)
 
I've never filtered before. All of you have me seriously thinking about it. Lol
 
chmuse said:
The way I see it it's like an extra $1.50 a vial and I'm on low dose sema. One vial 5mg vial lasts me like two months (depending on the brand/batch) I can afford it.
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Really, 1.50 is not much. Especially, with the price I used to pay with compound. What's 1.50 more? It might be worth a shot trying. It couldn't hurt.
 
Iamnew2this said:
Really, 1.50 is not much. Especially, with the price I used to pay with compound. What's 1.50 more? It might be worth a shot trying. It couldn't hurt.
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It's really a personal choice and I'm not going to judge anyone who chooses to go either way 🤷‍♀️ I've still never filtered because I keep saying 'I'll order before I need another vial' and then forgetting.
 
chmuse said:
It's really a personal choice and I'm not going to judge anyone who chooses to go either way 🤷‍♀️ I've still never filtered because I keep saying 'I'll order before I need another vial' and then forgetting.
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I totally understand you. I feel the same way. I don't filter, but I'm thinking that it just might be better than, not doing it.
 
Iamnew2this said:
I totally understand you. I feel the same way. I don't filter, but I'm thinking that it just might be better than, not doing it.
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At this point I'm just going to go buy a starter kit somewhere so I stop putting it off.
 
AndyPanda said:
As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.

I’ve never stored a vial for more than 5 days though.
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Isn't it a lot cheaper to buy bigger vials though ?
 
rkl123 said:
Isn't it a lot cheaper to buy bigger vials though ?
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Bigger vials? Do you mean higher milligrams?I suppose you save money if you have a lab freezer and will actually use it before it degrades. The highest milligram vials I have are T40 and have a bunch of T30 to get through so one vial lasts 10 days.
 
AndyPanda said:
You would want to put it into a new vial or you are wasting your time and energy.
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That doesn't make sense to me....are you suggesting the original vial wasn't sterile to begin with? If that's the case, it's not going to matter regardless.. Or are you concerned with residual?
 
Jfrick11 said:
That doesn't make sense to me....are you suggesting the original vial wasn't sterile to begin with? If that's the case, it's not going to matter regardless.. Or are you concerned with residual?
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yes, the original vial is most likely not sterile. they fail sterility tests often. Filtering with 0.22um filter makes the fluid sterile but it doesn't matter if you put it back into the same vial it came from.
 
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