Do You Filter Your Peptides Before Use?

[zpped]

Damn I missed all the fun.
As someone who does filter, I don't think its all that critical.

 

[AndyPanda]

I'm in NY.

People died. The elderly are STILL dying (though these are people who could have been taken out by flu, too.)

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

 

[AndyPanda]

Damn I missed all the fun.
As someone who does filter, I don't think it’s all that critical.

Best answer ever! You win the internet today. lol We’ve lost course.

 

[StonePny]

Woah I bet you really wow the kids at parties.

I’m a statistics and science guy who has worked in semiconductor production and bio-pharma production, what have you done?

Let’s break what you said down and what you are implying by linking this study:
- Contamination implies “you are going to have an adverse reaction.” Incorrect on so many levels. Cars can go without an oil change for 30,000 miles and not suffer an adverse reaction. Is that a good idea? No, can you predict each individual responses? No.

- as it’s obvious you don’t really understand statistically relevant versus data, your phone has a HUGE level of contamination, in fact all of our phones probably do (that’s just data). People who have a reaction to this contamination (statistically relevant) is most likely well below 1%, which could be caused by lower immune systems, worse hygiene, already sick, etc.

filters are designed to prevent statistically relevant outcomes that apply to the filtering process (small bacteria/foreign materials existing in the source). Filters are NOT designed to stop re-sticking a vial and the contamination subsequent to that.

The rules of:
- Don’t use BAC water past 30/60 days once punctured
- Don’t use peptides past 30/60 days once reconstituted + punctured
- Clean the cap prior to every stick with alcohol pad

Those rules exist to lower your chance of a statistically relevant event related to contamination which is NORMAL in use.

Many people go well beyond these limits and are just fine, because we make rules based on high risk aversion.

Many fantastic independent studies in this community have been done even on peptide degradation that drastically go over recommended maximum frozen storage levels (2-3 years) proving that risks are relatively low on using product frozen for even 4-5 years. I bet by your thought process there is a 25% chance those people must have keeled over by now

The cost/benefit analysis was a clever illustration, I liked it!
I do think that person needs to get a refund on the charm school he went to.

 

[chmuse]

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

I can absolutely confirm it was not only patients with preexisting conditions when it first happened. But the strains out there are milder now as far as I know.

 

[StonePny]

As a recovering intravenous meth addict (sober 19 years). I can assure you, the filter is a waste of money. We used a spoon and pulled off the cotton from a q-tip with our bare fingers as a filter. You'll be just fine. LOL

I shouldn't laugh, but I can tell you're really funny!
Also, well done on the 19 yrs

 

[hexagonal]

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

Being overweight is one of those pre-existing conditions. Huge comorbidity risk with covid.

A hell of a lot of the world, and America in specific, is overweight. Shit, that's why I'm here.

 

[Cndmk]

The cost/benefit analysis was a clever illustration, I liked it!
I do think that person needs to get a refund on the charm school he went to.

I appreciate that, I think many people just assume that statistics and probabilities are some kind of nebulous concept that rarely provoke a direct answer, but if you take those concepts and apply real life numbers, the math can give you an EXACT yes/no answer.

There is still some interpretation to what I stated as to how often a vial is “dangerous” to use (this information would be VERY hard to obtain), but I doubt the numbers are far off from reality in terms of risk (I was probably being a bit conservative)

There is a fantastically written book on this, Algorithms to live by, that gives people concrete answers to basic questions of life. Things like “how long should I search for a house when I’m interested in buying”, there is an actual number of days that has the highest probability of being ideal based on when you want to be done searching.

 

[Jfrick11]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?

 

[Skidude]

When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?

Here is a popular how-to

 

[Jfrick11]

Ahhh, ok thanks . The filter has a spot for the needle...there you go

 

[AndyPanda]

When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?

You would want to put it into a new vial or you are wasting your time and energy.

 

[keangkong]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

I don't filter my peptides. Janoshik wrote: "Given what I've seen around during all the years, infections from subcutaneous injections are... extremely rare even with the worst of practices."

 

[flyingheart4]

I don't filter my reconstituted peptides, but I will add some extra BA sometimes. The BA is what's going to safeguard you more than filtering from bacteria. I do filter the stuff I homebrew. Filtering is really more for particles/materials than could have fallen into the solution and BA it to make sure there is no bacteria. Filtering does add an extra layer of safety which isn't a bad thing. The type and size of filter listed is spot on the best way to go if you are going to start filtering your peptides.

As for refrigerating BAC. Hospira recommends against refrigeration even when opened. I however do refrigerate after opening. Hospitals don't refrigerate it either, but like I said above sometimes I will add a little more BA to make it like 1% instead of .9% for a little extra safety. Also, BAC is cheap. Don't use shit Amazon BAC. Buy Hospira BAC. I personally have like 20 3ml bottles at all times. If I really doubt something I just throw it away to lower risk.

What olis BA? And how much do you add to a vial?

 

[AndyPanda]

As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.

I’ve never stored a vial for more than 5 days though.

 

[zpped]

As I’m known for as of late, I fear I am about to add more contention…

I reconstitute my Tirzepatide with Polybcomplex from a glass ampule that I do not filter. I am taking the B Complex daily anyway, so when Tirz day comes I recon with it. Some pharmacies compound it with B-12, so I feel safe combining them, and it contains .9% BA. It’s saving me resources via a syringe and the Bacteriostatic Water. I have been looking into the blunt filter needles that @chmuse told me about for drawing from ampules but haven’t committed just yet because they are a bit pricey and I can only find packs of 100.

You're definitely injecting yourself with micro shards of glass.

 

[AndyPanda]

You're definitely injecting yourself with micro shards of glass.

Maybe, but I’ve not had any reactions.

 

[zpped]

Maybe, but I’ve not had any reactions.

No maybe about it. There is tons of literature on it. You're not going to die but the effects can be cumulative when you start getting weird muscle pain 10 years from now.

 

[AndyPanda]

No maybe about it. There is tons of literature on it. You're not going to die but the effects can be cumulative when you start getting weird muscle pain 10 years from now.

I have shrapnel in my leg. It can’t be worse than that.

 

[Skidude]

I have shrapnel in my leg. It can’t be worse than that.

It can be Shrapnel and glass shards...

Forgive me, I could not resist..