Do You Filter Your Peptides Before Use?

[Calm Logic]

Personal story: when I first tried "pinning" myself with Polaris's peptides, I gave myself cellulitis (redness, heat, tenderness, indurated region of about 10x6cm directly at the site of injection) which I had to treat with 5days of antibiotics. I thought I had just broken sterile procedure, so the next week I went extra hard on using lots of new alcohol swabs on rubber tops, on my skin, marked my skin pinning site w/marker etc. Within 24hrs I got another hard, red, hot site of cellulitis and again I had to treat myself with antibiotics to clear it. I blamed Polaris for likely contaminated product and thought about contacting them to complain, but then realized the fault was mine for not filtering the products. Ever since then SYRINGE FILTERING ABSOLUTELY for everything and problems solved.

Not likely cellulitis (if self-diagnosed), per Google Gemini:

 

[exploitedworkerbee]

Wearing a mask in public is a sign of submission to propaganda and mental illness. lol

Believing this is a sign of unwarranted self-righteousness and political indoctrination. Some people need to avoid getting sick for lots of reasons. For example during flu season I’ll mask in public for a few days/week before a big trip because I don’t want to be sick on my vacation. If you see me with my mask and automatically believe that it’s because I’ve submitted to propaganda when in fact I’m just a guy controlling what I can so I don’t feel like shit on my holiday, who is the one that has in fact been indoctrinated? You seem really, really lodged deep in your right wing media ecosystem, maybe this example will help you to see that.

 

[gpwdeux]

I cannot argue your response, but please take another look at my post and see I was comparing Russian roulette to using illicit street drugs with questionable provenance, potentially filled with fentanyl. All the while reconstituting with backwash water bottle, with dirty hands and spoon, and a used needle.
While high as shit in a trap house.

Now that I have done a better job of explaining the problem, would you say the statistical analogy is at least in the realm of comparison, not to mention unadvisable?

as for the rest of your post, I agree completely.

PS:
You may appreciate one of my favorite quotes:
57.6% of all statistics are wrong.

I was never good with word problems, always skimmed them. Now that you clarified, not only must I concur with the premise, but also agree that it is definitely within the realm of comparison, and , mathematically at least, unadvisable.

 

[AndyPanda]

Believing this is a sign of unwarranted self-righteousness and political indoctrination. Some people need to avoid getting sick for lots of reasons. For example during flu season I’ll mask in public for a few days/week before a big trip because I don’t want to be sick on my vacation. If you see me with my mask and automatically believe that it’s because I’ve submitted to propaganda when in fact I’m just a guy controlling what I can so I don’t feel like shit on my holiday, who is the one that has in fact been indoctrinated? You seem really, really lodged deep in your right wing media ecosystem, maybe this example will help you to see that.

Everything you just accused me of being, is reflected in your own post. Do you want to be the pot, or the kettle? But, fair enough. I’m not mad at you. We all do what we think is best for ourselves.

 

[tncc_rn]

We all do what we think is best for ourselves.

"This is the way......"

 

[hexagonal]

Everything you just accused me of being, is reflected in your own post. Do you want to be the pot, or the kettle? But, fair enough. I’m not mad at you. We all do what we think is best for ourselves.

What is political about wearing a mask to not spread germs?

This has been standard in places where infection risks are an issue or countries that are more conscientious about this since long before covid. This shouldn't be an issue that causes political division - if you're coughing or sneezing, wear a mask 'cause there's no reason for anybody to get more of those germs on you no matter what way they vote in the booth in november.

 

[exploitedworkerbee]

We all do what we think is best for ourselves.

Where does projecting (and announcing) your false, politically-driven narrative about other peoples’ motivations for masking fit into this laissez-faire worldview? I’m having trouble squaring that circle.

 

[AndyPanda]

This is not an argument worth engaging in. You can’t change people’s minds that feel the way you do, and that’s ok. To be clear, I am not “far right.” I think anyone who is “far” any direction needs to learn how to think for themselves and stop watching their favorite media outlet.

 

[exploitedworkerbee]

This is not an argument worth engaging in. You can’t change people’s minds that feel the way you do, and that’s ok. To be clear, I am not “far right.” I think anyone who is “far” any direction needs to learn how to think for themselves and stop watching their favorite media outlet.

If you don’t want to argue about masking then don’t make false, insulting, blanket statements about why people wear them. I provided a very reasonable example of why you might see me masking in public from my own actual lived experience—spending thousands on a trip I don’t want to mess up by coming down with the flu or cold or Covid—and instead of internalizing and engaging with that you’re digging in on your weird false belief. I encourage you to step back and reflect on that reaction, considering your initial accusation that they’re a sign of having fallen prey to propaganda. Agreed that it’s not a conversation we should continue but it saddens me when people are so unable to view their irrational beliefs with perspective even when clear evidence is provided.

 

[AndyPanda]

If you don’t want to argue about masking then don’t make false, insulting, blanket statements about why people wear them. I provided a very reasonable example of why you might see me masking in public from my own actual lived experience—spending thousands on a trip I don’t want to mess up by coming down with the flu or cold or Covid—and instead of internalizing and engaging with that you’re digging in on your weird false belief. I encourage you to step back and reflect on that reaction, considering your initial accusation that they’re a sign of having fallen prey to propaganda. Agreed that it’s not a conversation we should continue but it saddens me when people are so unable to view their irrational beliefs with perspective even when clear evidence is provided.

Ok. Your masks do not do anything. They are ineffective and make you look silly. Take some Vitamin C and Zinc. Breath freely in the air the way god intended and don’t cry and complain when other people don’t conform to your belief system.

 

[Cndmk]

Ok. Your masks do not do anything. They are ineffective and make you look silly. Take some Vitamin C and Zinc. Breath freely in the air the way god intended and don’t cry and complain when other people don’t conform to your belief system.

I didn't care about your beliefs in things, and I'm going to assume you are misinformed. If you disagree with the source below, then you are directly misleading, which I hold to the same level as trying to do harm unto others:
CDC - Do Masks Prevent Respiratory Viruses

Vitamin C can provide an effect on respiratory viruses, so that recommendation makes sense.
Vitamin - C study

If you were misinformed and needed some education on the topic, that is TOTALLY cool, but if you are trying to convince people against medical understanding on masking, then you should cease that crap right now. We have enough tik-tok "medical geniuses" at this point that Measles is making a dang comeback, and we need LESS misinformation, not more in this world.

 

[exploitedworkerbee]

we are doomed

 

[AndyPanda]

we are doomed

You are. You are easy to identify too. You are the victims in waiting with your masks donned. 😘

 

[Guyroscoe]

If you read any of my posts you would know that this was written without malice in an attempt to clear the air as opposed to judge.

I have a great sense of humor.. lol, it just might be reserved for other subject matter with less potential for harm.

However, when we are dealing with health issues, involving novices who potentially will make life changing decisions, based on knowledge obtained here; I feel like it is worth setting the record straight for posterity.

Please accept my apology if my post offended you.

No offense at all.

 

[hexagonal]

Ok. Your masks do not do anything. They are ineffective and make you look silly. Take some Vitamin C and Zinc. Breath freely in the air the way god intended and don’t cry and complain when other people don’t conform to your belief system.

Masks are less effective for preventing infection being spread to you, but they still do have a proven effect.

They are significantly more effective at blocking the transmission of particles from people that are infectious. I don't mask up when I'm not sick, but I 100% do if I think there's any chance that I am.

It's courtesy, not a political statement.

 

[AndyPanda]

I didn't care about your beliefs in things, and I'm going to assume you are misinformed. If you disagree with the source below, then you are directly misleading, which I hold to the same level as trying to do harm unto others:
CDC - Do Masks Prevent Respiratory Viruses

Vitamin C can provide an effect on respiratory viruses, so that recommendation makes sense.
Vitamin - C study

If you were misinformed and needed some education on the topic, that is TOTALLY cool, but if you are trying to convince people against medical understanding on masking, then you should cease that crap right now. We have enough tik-tok "medical geniuses" at this point that Measles is making a dang comeback, and we need LESS misinformation, not more in this world.

People not washing their hands is a bigger health risk than someone breathing has ever been. Here, I can use Google too. People are getting measles because they are not getting proven, non faddish vaccinations. Another negative side effect from the impact that making people get COVID vaccinations has had. It’s blowback. People fail to realize that coronaviruse was nothing new in 2019. The good old fashioned Flu is responsible for more deaths than COVID or measles. Wash your hands, with soap and water. The best heath advice ever given.

 

[rkl123]

People not washing their hands is a bigger health risk than someone breathing has ever been. Here, I can use Google too. People are getting measles because they are not getting proven, non faddish vaccinations. Another negative side effect from the impact that making people get COVID vaccinations has had. It’s blowback. People fail to realize that coronaviruse was nothing new in 2019. The good old fashioned Flu is responsible for more deaths than COVID or measles. Wash your hands, with soap and water. The best heath advice ever given.

In this regard you're quite wrong. The broad classes of coronaviruses existed. This one was quite exotic, like nothing our immune systems had seen yet. Like look up the images and video from India when delta variant hit in the tail end of the pandemic.

In general Covid has had higher kill rates than flus in immunocompromised and elderly.

Pre vaccine and pre exposure to initial covid, was very much a roll of the dice. Most young people would roll well. Most old people would roll poorly (look up the horrific scenes New York went through with ventilator shortages). A small minority of younger adults would have their lives quite ruined by it. I know one.

 

[AndyPanda]

In this regard you're quite wrong. The broad classes of coronaviruses existed. This one was quite exotic, like nothing our immune systems had seen yet. Like look up the images and video from India when delta variant hit in the tail end of the pandemic.

In general Covid has had higher kill rates than flus in immunocompromised and elderly.

Pre vaccine and pre exposure to initial covid, was very much a roll of the dice. Most young people would roll well. Most old people would roll poorly (look up the horrific scenes New York went through with ventilator shortages). A small minority of younger adults would have their lives quite ruined by it. I know one.

You are probably right. I personally don’t know a single person who died, or was related to anyone else who died from COVID. I don’t take for granted that my experience is probably a luxury.

 

[penewbie]

You are probably right. I personally don’t know a single person who died, or was related to anyone else who died from COVID. I don’t take for granted that my experience is probably a luxury.

Not knowing anyone who died from COVID is not uncommon.

But not knowing anyone who is related to someone who died from COVID?

There has been over a million COVID deaths in America.
I think either you don't know many people at all, or you don't know a lot about the relatives of the people you do know.

 

[chmuse]

You are probably right. I personally don’t know a single person who died, or was related to anyone else who died from COVID. I don’t take for granted that my experience is probably a luxury.

I'm in NY.

People died. The elderly are STILL dying (though these are people who could have been taken out by flu, too.)

 

[zpped]

Damn I missed all the fun.
As someone who does filter, I don't think its all that critical.

 

[AndyPanda]

I'm in NY.

People died. The elderly are STILL dying (though these are people who could have been taken out by flu, too.)

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

 

[AndyPanda]

Damn I missed all the fun.
As someone who does filter, I don't think it’s all that critical.

Best answer ever! You win the internet today. lol We’ve lost course.

 

[StonePny]

Woah I bet you really wow the kids at parties.

I’m a statistics and science guy who has worked in semiconductor production and bio-pharma production, what have you done?

Let’s break what you said down and what you are implying by linking this study:
- Contamination implies “you are going to have an adverse reaction.” Incorrect on so many levels. Cars can go without an oil change for 30,000 miles and not suffer an adverse reaction. Is that a good idea? No, can you predict each individual responses? No.

- as it’s obvious you don’t really understand statistically relevant versus data, your phone has a HUGE level of contamination, in fact all of our phones probably do (that’s just data). People who have a reaction to this contamination (statistically relevant) is most likely well below 1%, which could be caused by lower immune systems, worse hygiene, already sick, etc.

filters are designed to prevent statistically relevant outcomes that apply to the filtering process (small bacteria/foreign materials existing in the source). Filters are NOT designed to stop re-sticking a vial and the contamination subsequent to that.

The rules of:
- Don’t use BAC water past 30/60 days once punctured
- Don’t use peptides past 30/60 days once reconstituted + punctured
- Clean the cap prior to every stick with alcohol pad

Those rules exist to lower your chance of a statistically relevant event related to contamination which is NORMAL in use.

Many people go well beyond these limits and are just fine, because we make rules based on high risk aversion.

Many fantastic independent studies in this community have been done even on peptide degradation that drastically go over recommended maximum frozen storage levels (2-3 years) proving that risks are relatively low on using product frozen for even 4-5 years. I bet by your thought process there is a 25% chance those people must have keeled over by now

The cost/benefit analysis was a clever illustration, I liked it!
I do think that person needs to get a refund on the charm school he went to.

 

[chmuse]

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

I can absolutely confirm it was not only patients with preexisting conditions when it first happened. But the strains out there are milder now as far as I know.

 

[StonePny]

As a recovering intravenous meth addict (sober 19 years). I can assure you, the filter is a waste of money. We used a spoon and pulled off the cotton from a q-tip with our bare fingers as a filter. You'll be just fine. LOL

I shouldn't laugh, but I can tell you're really funny!
Also, well done on the 19 yrs

 

[hexagonal]

My understanding is that most of the people who died had some preexisting condition that was complicated by the virus. Unlike a previous accusation on here, I know a lot of people from all of the Country. As selfish as it sounds, I work in an industry that never slowed down during the “pandemic” and I recall several of my coworkers and employees using COVID as an excuse to miss work, only to be seen on Facebook doing anything but suffering. Maybe I’m just jaded.

Being overweight is one of those pre-existing conditions. Huge comorbidity risk with covid.

A hell of a lot of the world, and America in specific, is overweight. Shit, that's why I'm here.

 

[Cndmk]

The cost/benefit analysis was a clever illustration, I liked it!
I do think that person needs to get a refund on the charm school he went to.

I appreciate that, I think many people just assume that statistics and probabilities are some kind of nebulous concept that rarely provoke a direct answer, but if you take those concepts and apply real life numbers, the math can give you an EXACT yes/no answer.

There is still some interpretation to what I stated as to how often a vial is “dangerous” to use (this information would be VERY hard to obtain), but I doubt the numbers are far off from reality in terms of risk (I was probably being a bit conservative)

There is a fantastically written book on this, Algorithms to live by, that gives people concrete answers to basic questions of life. Things like “how long should I search for a house when I’m interested in buying”, there is an actual number of days that has the highest probability of being ideal based on when you want to be done searching.

 

[Jfrick11]

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

Hey everyone,

I've been diving deep into peptide handling protocols lately and wanted to start a discussion about something that seems simple but could make a huge difference in your research: Do you filter your reconstituted peptides before use, or do you use them as is?

Why This Matters:
For those just getting started with peptides, filtering might seem like an extra, unnecessary step. However, it's actually crucial for several reasons:

• Safety First: Unfiltered peptides may contain bacteria, particulate matter, or manufacturing residues that could cause infection, inflammation, or adverse reactions when used in research settings.
• Protecting Your Investment: Quality peptides aren't cheap! Using them without proper filtration is like buying premium fuel for your car but pouring it through a dirty funnel. You're compromising what you paid for.

The Real Value:
When you filter your reconstituted peptides through a proper 0.22 μm syringe filter, you're:

• Removing potentially harmful bacteria and particles that could cause injection site reactions or infections
• Maximizing the purity of your product, which directly translates to more reliable outcomes
• Protecting yourself from potential contaminants introduced during manufacturing or shipping

Beginner-Friendly Best Practices:
If you're new to this, here's what you need to know:

What to Buy:

• Get sterile 0.22 μm syringe filters (PES/Polyethersulfone filters are best as they don't absorb your peptides)
• Make sure you have sterile syringes to use with the filters
• Consider the volume you'll be filtering (smaller 4mm filters for under 4ml, larger 13mm filters for more)

Simple Process:

1. Draw your reconstituted peptide into a sterile syringe
2. Attach the filter to the syringe
3. Slowly push the liquid through the filter into your sterile storage vial
4. Label appropriately with contents and date

Remember that filtering is not just a "nice to have" but a fundamental step that separates proper research protocols from potentially dangerous shortcuts. The small investment in filters (usually $1-2 each) provides enormous value in terms of safety.

Has anyone here experienced noticeable differences between filtered and unfiltered peptides (especially with overseas purchases)? Or perhaps you've always filtered but weren't sure why it matters? I'd love to hear your experiences!

When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?

 

[Skidude]

When you say slowly push the liquid through the filter into your sterilized vial..... How do you do that.
When I reconstitute, I put back water into the vial I get from the supplier with a needle. If I withdraw the reconstituted solution, taje off the needle and put on the filter...... How do you get it back into the vial without a needle?

Here is a popular how-to