In Novo’s Own Words: Degradation of Amylin Analogs Such as Cagrilintide (and How to Test For It)

[secretweapon]

Could yall be confusing Cagri with Pramlintide

Here's something I wrote. Does it answer your question?

Cagrilintide retains the three proline substitutions that pramlintide made to human amylin to make it resistant to fibrillation, but it has four more improvements:

1. While pramlintide has a short half-life and must be dosed multiple times per day, researchers created a long-acting version that allows once-weekly dosing by adding a long fatty acid chain to the pramlintide molecule. The fatty acid allows cagrilintide to bind to albumin (a protein in the blood). When bound to albumin, cagrilintide is cleared from the body more slowly.
2. Researchers made cagrilintide resistant to a chemical reaction called deamination, which can break down the molecule, by replacing the asparagine amino acid (Asn) at position 14 with glutamic acid (Glu). Note: deamiNation is a distinctly different process from deamiDation (the latter leading to fibrillation).
3. They also made cagrilintide more soluble, meaning it dissolves more easily in water, by replacing valine (Val) with arginine (Arg) at position 17.
4. While pramlintide is a single-hormone agonist for amylin receptors, cagrilintide is a dual-hormone agonist for both amylin and calcitonin receptors. This is due to proline (Pro) being replaced by Tyrosine (Tyr) at position 37.

Those are the only four changes made to pramlintide to create cagrilintide. One important thing to note is that none of the changes made pramlintide more resistant to fibrillation or more stable at a higher pH. This means that any research on the pH requirements or fibrillation of pramlintide will also apply to cagrilintide.

 

[scarywood75]

View attachment 4065

Don't bother making these types of posts here, people are very closed mind as soon as you do things/think differently, even if it's for the better.

I'm a living proof of that too

 

[nurseratchit]

@secretweapon sorry who are you? You just joined today to post this, and your conclusion is that people who disagree with your assessment have ulterior motives?

I think he’s megalith. He’s been busy spreading this in multiple places.

 

[nurseratchit]

I

that was funny the other 10 times you said it in Discord.

It was funny the other 10 times you spewed this nonsense in Discord

 

[secretweapon]

I think he’s megalith. He’s been busy spreading this in multiple places.

Yeah, a handful of people still can't tell we have entirely different personalities, but most people have it figured out.

 

[secretweapon]

I think he’s megalith. He’s been busy spreading this in multiple places.

I literally just shared direct quotes from Novo Nordisk about their own drug. Which of their statements do you consider ?

 

[Yay-Z1]

View attachment 4065

Seems like this is what you and Megalith default to when anyone shares something different from y’all’s view. Have a good day, I had a nice laugh .

 

[Dee X]

Don't bother making these types of posts here, people are very closed mind as soon as you do things/think differently, even if it's for the better.

I'm a living proof of that too

You had ChatGPT inventing untested ways of reconstitution that didn't involve BAC, and decided to then have it write up a giant guide about how to do it as if it were proven in trials.

 

[DevynL90]

Seems like this is what you and Megalith default to when anyone shares something different from y’all’s view. Have a good day, I had a nice laugh .

i mean you shared a google AI summary as your rebuttal not to be rude but wtf were you expecting?

 

[scarywood75]

You had ChatGPT inventing untested ways of reconstitution that didn't involve BAC, and decided to then have it write up a giant guide about how to do it as if it were proven in trials.

This thread is getting spicy, let's bring some popcorn and watch

 

[secretweapon]

Seems like this is what you and Megalith default to when anyone shares something different from y’all’s view. Have a good day, I had a nice laugh .

Maybe you should stop defaulting to sharing misinformation?

 

[Yay-Z1]

i mean you shared a google AI summary as your rebuttal not to be rude but wtf were you expecting?

I got exactly what I expected

 

[scarywood75]

It's time to shine before an admin locks this thread again, adding to the drama is the only reason I'm commenting here, like all these comments lol

Nice write up btw

 

[DevynL90]

It's time to shine before an admin locks this thread again, adding to the drama is the only reason I'm commenting here, like all these comments lol

Nice write up btw

for the love of god do not lock this thread. whether he rubs you the wrong way or you disagree with his premise, this is a good thread topic.

 

[nurseratchit]

Y

Don't bother making these types of posts here, people are very closed mind as soon as you do things/think differently, even if it's for the better.

I'm a living proof of that too

I’ll admit to being close-minded to fear-mongering AI generated conspiracy propaganda.

 

[scarywood75]

for the love of god do not lock this thread. whether he rubs you the wrong way or you disagree with his premise, this is a good thread topic.

I'm all with ya buddy

 

[nurseratchit]

For the chemistry majors here’s research article about amylin and fibrils. For the TL;DR, yes fibrils were a concern which is why modifications have been made to cagri to suppress fibril development. You can scroll to the summary at the end if chemistry isn’t your thing.

https://doi.org/10.1021/acs.jmedchem.1c00565

 

[scarywood75]

Y

I’ll admit to being close-minded to fear-mongering AI generated conspiracy propaganda.

Oh no

 

[scarywood75]

You guys and girls had a rough week, it's time to let it all out

 

[nurseratchit]

I literally just shared direct quotes from Novo Nordisk about their own drug. Which of their statements do you consider ?

You seem to have a weird fibril fetish and are well known for it in the community. I’m pretty sure others have already tried to talk you down so I won’t waste my breath. I will refer you to my previous comment and cited article.