Putting Air in the syringe and into your vial.

To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill
I only recon in front of my flow hood, so all air going into a vial is clean.
I use pens as well.
 
I didn't used to inject air. And one some vials I would literally watch air bubbling in from a previous needle puncture as I was drawing out the next dose.

I now use pens, and inject air into the vial, then filter the 'tide into the cartridge, making the whole thing a non-issue.

Here's something that will really gross out germ-phobes. Guess how often on average a T1D change out the needles on their pens ?
I pointed this out a few weeks ago, in reference to that Fishermandude's post about never changing needles. I was talking to a 30+ yr nurse. NOBODY outside of the psycho-pep cult of doom is changing needles every shot on their own personal injections. According to her.

YMMV.
 
NOBODY outside of the psycho-pep cult of doom is changing needles every shot on their own personal injections. According to her.
That just doesn't sound right 😛. Are you saying they are drawing and injecting multiple shots with the same needle?!
 
To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill
I do not inject air just because I don't need to.
 
What the hell are you talking about? Please explain your logic, I'd love to hear it. Do you filter your stuff? You vent the new vial to help with pushing the reconstituted product into the new vial. All of the new vials I've used have no vacuum, so pushing 2-3ml of reconned product, the syringe will fight back.
You seem to be considering a very narrow use case. One that doesn't even apply to the current thread. The OP was about injecting air into a vial before drawing. Are you saying that drawing a substantial amount from a vial will not create a vacuum, which may make it difficult to draw (if not impossible)? Well, it does.

You can inject air or vent the vial. Either way, the air is getting in.
 
To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill
Personally I don’t test my stuff after I start using is, just basically inject until it’s cloudy. But never had an issue with anything clouding up before a reasonable time.
 
You seem to be considering a very narrow use case. One that doesn't even apply to the current thread. The OP was about injecting air into a vial before drawing. Are you saying that drawing a substantial amount from a vial will not create a vacuum, which may make it difficult to draw (if not impossible)? Well, it does.

You can inject air or vent the vial. Either way, the air is getting in.
I think he's saying that a new sterile, empty vial he adds a vent with filter on it and when he injects the liquid from the 1st vial, it's just pushing OUT the air that was inside.

He's not introducing air in his example. He's removing whatever was there to begin with.

Now, when he does that, his new vial won't have a vacuum on it so he shouldn't need to push air into a vial using his "move stuff to a new vial" method.

I think.
 
That just doesn't sound right 😛. Are you saying they are drawing and injecting multiple shots with the same needle?!
yup. Contextual note, I'm pretty sure she was referring to multiple-use autopens, similar to the manual pens that are common in our world. Not re-using a single-use diabetic syringe.

(puts on flame suit) I didn't bring enough needles to my remote location where I'm stationed for the next few months, and so now I'm "one of those" that don't change the needle on my pen every injection.
 
Personally I don’t test my stuff after I start using is, just basically inject until it’s cloudy. But never had an issue with anything clouding up before a reasonable time.
The cloudiness or particles intrigues me. I’ve got a few peps I’ve been using for three months and they both look great. Tirzep mainly. How long has it taken you to get a cloudy vial. I have a few friends that have been using the same vial for six months. I know everyone has an opinion on this and I think the grey market crowd does not have this issue. It’s the crowd that bought a 3 month pre mixed vial from a compounder and we paid 5x for it and we just can’t let it go. It’s funny how the learning and experience is almost identical for most here in this forum.

Bill
 
The cloudiness or particles intrigues me. I’ve got a few peps I’ve been using for three months and they both look great. Tirzep mainly. How long has it taken you to get a cloudy vial. I have a few friends that have been using the same vial for six months. I know everyone has an opinion on this and I think the grey market crowd does not have this issue. It’s the crowd that bought a 3 month pre mixed vial from a compounder and we paid 5x for it and we just can’t let it go. It’s funny how the learning and experience is almost identical for most here in this forum.

Bill
I typically run out of my Reta around week 8. Never been cloudy at that point. If I was taking lower doses I’m sure it would last longer though, I just run out of the compound before it clouds up.
 
I've based all of my kits on a 6 week maximum lifetime.

I'm kind of surprised to see so many people planning on much, much longer.
 
To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.
There's a test running on another server with air injected into vials with BAC daily that has shown no problems. That is the whole point of BAC.
 
There's a test running on another server with air injected into vials with BAC daily that has shown no problems. That is the whole point of BAC.
I believe you. But there a few that disagree. They say BAC water can’t prevent bacteria from forming. Only help. But bacteria can form in BAC water. I don’t have an issue adding air.

Bill
 
I have just made a simple video to let you see if air contamination really exists or not? Device counts bad particles in the air at standard room conditions with stable air flow. It is just a comparison next to a laminaire flow hood and in front of it. Counter can tell particles bigger than 2.5micron and hepafilter of the hood is 2micron. Decide yourself just by clicking on the link

Bad particles counter

It is on my google drive. Probably you have to download it first to see. It is an .mp4 video
 
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I mean nurses are taught to pull air into the syringe on day 1 in nursing school.
It is just to use the content immidiately. Not to leave air in the vial for a long time and they also learn at first day, not to leave any air in the syringe, before they inject it, not?
 
That just doesn't sound right 😛. Are you saying they are drawing and injecting multiple shots with the same needle?!
I think it is about pen usage with the same needle for a couple of times. No need to take risks for such a low cost, though some people do it. I gave klow to a friend in a pen together with 20 needles and told him to use a new needle each time. You can order it online or just go to a pharmacy and buy needles for mounjaro pen without any questions, I said. After 4 weeks he had 15 needles over out of 20. No problems at all, he says. To use good practices, he must get problems first. Nothing to do at all than hoping that he doesn't get any issues.
 
As I mentioned, I only push air into vial that I draw shots from. When reconstituting into a new empty vial, I vent to help filling the 2-3ml of reconned peptide in the new vial. Otherwise, sometimes it's hard to pull the liquid into the pin syringe. Zippy even mentioned this a while back.
 
I have just made a simple video to let you see if air contamination really exists or not? Device counts bad particles in the air at standard room conditions with stable air flow. It is just a comparison next to a laminaire flow hood and in front of it. Counter can tell particles bigger than 2.5micron and hepafilter of the hood is 2micron. Decide yourself just by clicking on the link

Bad particles counter

It is on my google drive. Probably you have to download it first to see. It is an .mp4 video
Great video! I love my flow hood! I use it for all sorts of fun hobbies.
 
I have just made a simple video to let you see if air contamination really exists or not? Device counts bad particles in the air at standard room conditions with stable air flow. It is just a comparison next to a laminaire flow hood and in front of it. Counter can tell particles bigger than 2.5micron and hepafilter of the hood is 2micron. Decide yourself just by clicking on the link

Bad particles counter

It is on my google drive. Probably you have to download it first to see. It is an .mp4 video
Did you get that from Amazon? I was looking at one that looks similar.
 
I have just made a simple video to let you see if air contamination really exists or not? Device counts bad particles in the air at standard room conditions with stable air flow. It is just a comparison next to a laminaire flow hood and in front of it. Counter can tell particles bigger than 2.5micron and hepafilter of the hood is 2micron. Decide yourself just by clicking on the link

Bad particles counter

It is on my google drive. Probably you have to download it first to see. It is an .mp4 video
What are the specifications of your equipment? I can't see the hood in the video: do you have a wider view?
 
Did you get that from Amazon? I was looking at one that looks similar.
No. I made the laminaire flow hood myself for less than €100. All material including particles counter bought from ikea at the department where they sell air ionisers and cleaners. Only 12V adaptor with potentiometer for regulating fan speed and PC fans were from Aliexpress. Reason behind making it self is not the cost. But the challenge behind making itself and seeing that it works. But I have checked the link you gave. It looks great for the price and it will sure do the job.
 
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What are the specifications of your equipment? I can't see the hood in the video: do you have a wider view?
A laminaire flow hood is not a rocket science. 2 micron hepafilter and carbon filter, 2x 12V pc cooloing fans, 12V speed regulating adapter and a plastic box is all you need. Particles counter, box, hepa and carbon filters are from IKEA. Adapter and fans are from Aliexpress.

It is not for professional use and doesn't have to be more than that. Yes, it can be much easier and cheaper than you can imagine. Reconstituting your vials and filling cartridges in front of it gives you some peace of mind at least. You must use it at an environment free of external air flow and pets. Also sanitise all material you use by spraying them with %70 isopropyl alcohol through an atomiser. %70 is more efficient than %100 because of evoporating speed.

WhatsApp Image 2026-06-21 at 16.07.24.webp
 
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A laminaire flow hood is not a rocket science. 2 micron hepafilter and carbon filter, 2x 12V pc cooloing fans, 12V speed regulating adapter and a plastic box is all you need. Particles counter, box, hepa and carbon filters are from IKEA. Adapter and fans are from Aliexpress.

It is not for professional use and doesn't have to be more than that. Yes, it can be much easier and cheaper than you can imagine. Reconstituting your vials and filling cartridges in front of it gives you some peace of mind at least. You must use it at an environment free of external air flow and pets. Also sanitise all material you use by spraying them with %70 isopropyl alcohol through an atomiser. %70 is more efficient than %100 because of evoporating speed.

View attachment 28119

A smart DIY home lab 🙂

I hadn't thought of a box like that. I still have some left over from my last move \o/

I was looking at the acrylic sheets... It's much simpler.

Just a practical question: where do you pass your hands through?
 
A smart DIY home lab 🙂

I hadn't thought of a box like that. I still have some left over from my last move \o/

I was looking at the acrylic sheets... It's much simpler.

Just a practical question: where do you pass your hands through?
I don't have to pass my hands through anywhere. Just placing it on a sanitized glass table, placing a sanitised grill in front of it and processing in front of it where I have placed the counter on the video. Area up to 20-25cm in front of it is safe. Checked it by placing the counter at different spots and distances. Check the amazon link BNLFL gave to get an idea about the grill I use and usage of the hood
Did you get that from Amazon? I was looking at one that looks similar.
You must put your hands through holes, if you use a still air box which much simpler, cheaper but less thrusted is. Just google Still Air Box.

By the way, if you decide to make it yourself, place the fans to upper side or to a side wall. Not across the fillter. Air becomes laminaire because of the pressed air inside. Not by directly blowing on the filter. Fan speed regulator is also needed to find the optimal air speed to get good results. Too much or too little air pressure won't work good.
 
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