Tirz Freezing/Defrosting

[thatkatmat]

In a podcast interview Peter North or maybe it was Pete Magic said he had hgh he forgot about in a garage for years that tested fine. Tirz will likely last decades frozen. Peptides are not as fragile as reported, you can shake them and squirt directly with bac., no need to baby it and drizzle down the sides of the vial. I belive you said reconstituted vials so refrigerate don't freeze. (Not you Zipp, meant to respond to @thatkatmat , sorry.)

I watched that video, was quite eye opening! I was referring to reconstituting it, then freezing it once to use again later. Most everything I've read says don't do it. But on this forum there seems to be some research stating otherwise.

 

[Airborne Daddy]

I watched that video, was quite eye opening! I was referring to reconstituting it, then freezing it once to use again later. Most everything I've read says don't do it. But on this forum there seems to be some research stating otherwise.

They thought u meant unconstituted. No one will tell you to freeze a liquid.

 

[indolent]

No one will tell you to freeze a liquid.

false

 

[zpped]

They thought u meant unconstituted. No one will tell you to freeze a liquid.

I will, under certain circumstances.

 

[thatkatmat]

I will, under certain circumstances.

May I ask, which sort of circumstances?
A member in a similar thread said they're going to try it and report back, but ended up not reporting back. I joined peppy's but I it looks like it could be a while until I gain access to that info...
I guess the only thing I would lose is a little product and a day or 2 of being hungry if it doesn't work...I mean, before GLP1's I was hungry for the better part of 50 years...lol
Cheers and thanks again!

 

[woundcarping]

Tirz is apparently a robust peptide. If I was freezing liquid I would want the bottle to be fresh vs closer to the discard date. I’d want fresher BAC. I’d freeze it once and thaw it once, if I had a 120mg vial I might would portion it out into more useful vial sizes so I could thaw what I needed.

I’d be more inclined to store in a vacuum based thermos to give time if the power went out… if you want to get more aggressive, put the thermos in a quality cooler and fill the cooler with thermal mass, in a deep freezer (-40°). Think the thermos in a yeti cooler full of frozen water.

You could put tattle tales in to track if/when the temp got above the freezing point.

Then again, it’s just Tirz, freezer space with a bunch of extra layers could cost more.

 

[zpped]

May I ask, which sort of circumstances?
A member in a similar thread said they're going to try it and report back, but ended up not reporting back. I joined peppy's but I it looks like it could be a while until I gain access to that info...
I guess the only thing I would lose is a little product and a day or 2 of being hungry if it doesn't work...I mean, before GLP1's I was hungry for the better part of 50 years...lol
Cheers and thanks again!

woundcarping's first paragraph sums up my thoughts. I wouldn't bother with the second.

I'd also freeze reta the same way if I needed to.

 

[thatkatmat]

woundcarping's first paragraph sums up my thoughts. I wouldn't bother with the second.

I'd also freeze reta the same way if I needed to.

Awesome, Good to know, I'm gonna research it myself now...BTW, with your help i was able to get on peppy's and find some very interesting and corroborating information. As well as some info on Cagri that had been looking for..Thanks again!

 

[Airborne Daddy]

false

Elaborate perhaps? What reconstituted peptides do you freeze?

 

[Gr33dyOctopus]

Elaborate perhaps? What reconstituted peptides do you freeze?

I mean, I've been freezing my Reta and Tirz since I've been buying huge bottles. Divvying into smaller bottles, freezing And thawing those for myself and other rats, they seem to work fine??

 

[indolent]

Elaborate perhaps? What reconstituted peptides do you freeze?

I reconstitute a tirzepatide kit in one go, and move each vial to the fridge for its 4-6 weeks of active use.

I don't transfer to empty vials because I don't trust their sterility, and don't want to invest in lab-grade equipment to properly sterilize them. My pressure-cooker is for proteins.

 

[john_askins]

I mean, I've been freezing my Reta and Tirz since I've been buying huge bottles. Divvying into smaller bottles, freezing And thawing those for myself and other rats, they seem to work fine??

so you did reconstitute and then freeze? I reconstituted a vial when I got them in the mail and I'm just now getting to the point of needing it (ran out of name brand). I had planned to test if it still works after freezing it since my compounding pharmacy suggested freezing theirs.

 

[john_askins]

I reconstitute a tirzepatide kit in one go, and move each vial to the fridge for its 4-6 weeks of active use.

I don't transfer to empty vials because I don't trust their sterility, and don't want to invest in lab-grade equipment to properly sterilize them. My pressure-cooker is for proteins.

my previous message was actually for you, quoted the wrong person

so you did reconstitute and then freeze? I reconstituted a vial when I got them in the mail and I'm just now getting to the point of needing it (ran out of name brand). I had planned to test if it still works after freezing it since my compounding pharmacy suggested freezing theirs.

 

[indolent]

my previous message was actually for you, quoted the wrong person

so you did reconstitute and then freeze? I reconstituted a vial when I got them in the mail and I'm just now getting to the point of needing it (ran out of name brand). I had planned to test if it still works after freezing it since my compounding pharmacy suggested freezing theirs.

For tirzepatide, freezing once, then refrigerating, hasn't been shown in laboratory studies to degrade the content mass, which basically means "works."

There are questions about sterility however, with some people saying that the benzyl alcohol in the bac water can separate out when frozen. I'm not aware of lab tests for sterility with freeze/thaw/refrigerate.

My assessment, for my own use, is that I'm not worried about that...each of us has to gauge comfort level with risks independently.

 

[thatkatmat]

I've read that sterility would be the one thing you would worry about when freezing once and so perhaps try to use the thawed aliquot within 14-21 days, but, YMMV

 

[zpped]

I've read that sterility would be the one thing you would worry about when freezing once and so perhaps try to use the thawed aliquot within 14-21 days, but, YMMV

That doesn't make any sense.

 

[cldfront]

I've talked about this before, but when I first started in September of 2024 I purchased from a medispa/compounding pharmacy that sent me a vial of reconstituted Tirz that I was specifically instructed to "keep frozen", thaw on my counter once a week for drawing a dose, and then put it back in the freezer. It was a three month supply (side note, paid $3000 for it) and it worked so well that I knew I had to come up with a way to afford this medication forever and the rest is history.

 

[thatkatmat]

That doesn't make any sense.

I meant to say stability, not sterility. Post thaw stability could decrease slightly.

 

[indolent]

I meant to say stability, not sterility. Post thaw stability could decrease slightly.

What does stability mean in this context?

If = degradation, that's been tested for tirz and the decrease in mass is negligible, even after two (three?) freeze/thaw cycles.

 

[thatkatmat]

I was in fact referring to degradation, as I understand it , it's not because it suddenly “goes bad.” but rather, the freeze thaw cycle could introduce a tiny structural stress event
That possible stress reduces confidence slightly to where you might want to use conservative buffers. Maybe try to use it in 3 weeks rather than the standard 4... But I did also say "YMMV". I mean, some people use refrigerated peptides for months.

 

[randompersonrandom]

I'm really strongly considering @indolent 's recon-a-kit-and-freeze method. I'm down to my last 75 mg of compounded (frozen), and then I start on my mostly-gyc stashes. A kit of 30 mg would 37 weeks, and I don't know that I'd love the idea of ten punctures in a 30 ml bottle of bac over that amount of time. But if I recon it all and filter into five cartridges of 60 mg each (2.73 ml each), purge the air, and into the freezer, that should be enough wiggle room to keep the freezing from popping the cartridge bottom out. And I've frozen carts before and never had a blowout.

 

[gothsoup]

I'm new here so excuse the silly question, but after reading this entire thread I'm not sure I have an answer to it. I have a tirz kit and BAC water. They're supposed to last me for 30 weeks. Is it okay to reconstitute then freeze, and thaw the reconstituted vials as I need them? I'm a bit confused and overwhelmed 🙃

 

[Grogu]

I'm new here so excuse the silly question, but after reading this entire thread I'm not sure I have an answer to it. I have a tirz kit and BAC water. They're supposed to last me for 30 weeks. Is it okay to reconstitute then freeze, and thaw the reconstituted vials as I need them? I'm a bit confused and overwhelmed 🙃

The overwhelming sentiment in this thread is that many people have had success reconstituting tirzepatide and then freezing and thawing those vials as needed. The general thought is that tirzepatide is a robust peptide. What part is causing you confusion?

 

[CNCCurrency]

The overwhelming sentiment in this thread is that many people have had success reconstituting tirzepatide and then freezing and thawing those vials as needed. The general thought is that tirzepatide is a robust peptide. What part is causing you confusion?

Agreed, I believe someone stated early in this thread that tirz is the cockroach of peptides

 

[skeptick]

I'm new here so excuse the silly question, but after reading this entire thread I'm not sure I have an answer to it. I have a tirz kit and BAC water. They're supposed to last me for 30 weeks. Is it okay to reconstitute then freeze, and thaw the reconstituted vials as I need them? I'm a bit confused and overwhelmed 🙃

I'm new here so excuse the silly question, but after reading this entire thread I'm not sure I have an answer to it. I have a tirz kit and BAC water. They're supposed to last me for 30 weeks. Is it okay to reconstitute then freeze, and thaw the reconstituted vials as I need them? I'm a bit confused and overwhelmed 🙃

Hey Goth, How have you determined how much Tirz you have and how is it to last you 30 weeks? I agree with these others that there are a number of folks on this board who speak of recon/transfer to vials/freeze till needed...

 

[indolent]

I'm new here so excuse the silly question, but after reading this entire thread I'm not sure I have an answer to it. I have a tirz kit and BAC water. They're supposed to last me for 30 weeks. Is it okay to reconstitute then freeze, and thaw the reconstituted vials as I need them? I'm a bit confused and overwhelmed 🙃.

I wouldn't repeatedly thaw/refreeze any one particular reconstituted vial, if that's what you're asking. Thaw, then refrigerate, the active-use reconned vial.

 

[gothsoup]

I wouldn't repeatedly thaw/refreeze any one particular reconstituted vial, if that's what you're asking. Thaw, then refrigerate, the active-use reconned vial.

The idea is to reconstitute all vials, then freeze, and only thaw once when it's time to use a vial.

 

[designing677]

I reconstitute a tirzepatide kit in one go, and move each vial to the fridge for its 4-6 weeks of active use.

I don't transfer to empty vials because I don't trust their sterility, and don't want to invest in lab-grade equipment to properly sterilize them. My pressure-cooker is for proteins.

What do you find is the advantage of doing it this way, rather than reconstituting a vial at a time, immediately prior to use?

 

[thatkatmat]

I took a 60mg (actual 69mg) vial of Tirz, reconstituted it with Hospira Bac and split it into 3 vials and froze it. I'll pull one in a couple days and give it a go before this weekend and report back.

I'm fairly certain it will be fine after reading over and over again how robust it is. I've read that cagrinlintide will tolerate it even better than Tirz. Reta might be a little more fussy but if handled correctly should be good without much degradation.

 

[indolent]

What do you find is the advantage of doing it this way, rather than reconstituting a vial at a time, immediately prior to use?

Time-convenience; less waste of 30 mL opened bac vial.

The consumer convenience factor is why pharma companies have made trillions from extended-release pills, and why they're making trillions from weekly rather than daily GLP-1 injectables, and why they're developing monthly GLP-1 injectables.