I avoid Amazon for syringes, BAC or reconstitution solution, but why would you avoid Amazon for the sterility vials? I buy the Ks-Tek sterile vials I use there. Each individually wrapped and they come in packs of 10.
Syringe Filter
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moonpies4misfits
Advanced Researcher
A number of reviews saying they leaked, had particles floating in them, poorly crimped caps. The stopper is brittle, doesn't self heal, splits, and pieces break off into the vial. Just look at all the 1, 2, and 3 star reviews on them.
The few I have used are absolutely fine.
That whole paper is complete garbage science. Itās written by one of the prominent names in the antivax/COVID misinformation movement. The way he came up with the 250k number is absolutely ridiculous and was widely disputed after publication.
Just FYI!
I didnāt even open the url. The number alone is enough to call bullshit
zpped
Advanced Researcher
There were a few questions here I'll answer
peptest sells tisch filters which you can buy directly from tisch in pre-sterilised packets but they aren't always in stock and they only sell by the 100.
scientificfilters.com
The cobetter filters on amazon are also considered good but they only have 13mm if you really want 4mm.
the things that matter about being sterile are the 1) filter 2) the needle after the filter and 3) the vial/cartridge
If you want high quality syringes/needles/vials they can be found at lot of medical/lab supply companies that sell online.
peptest sells tisch filters which you can buy directly from tisch in pre-sterilised packets but they aren't always in stock and they only sell by the 100.
Syringe Filters
High quality products. Huge selection. Order online, call, or request a quote. Large selection of material types, housing sizes, and micron sizes. Expert advice. Samples available.
scientificfilters.com
The cobetter filters on amazon are also considered good but they only have 13mm if you really want 4mm.
the things that matter about being sterile are the 1) filter 2) the needle after the filter and 3) the vial/cartridge
If you want high quality syringes/needles/vials they can be found at lot of medical/lab supply companies that sell online.
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Trying not to post any 2020+ study estimates bc they go much higher for the estimates (even with COVID being split away into it's own category by CDC).
To be clear, this is totally not a hill I'm willing to die on. If I'm incorrect with the info I have found, please show otherwise since I want to know the proper info.
BD are lousy and I went to such crazy efforts to source them and paid too much and I keep going back to my Easy Touch syringes!
moonpies4misfits
Advanced Researcher
They were a bit more expensive at TDS but not too crazy. Still not worth it though. My new box of EasyTouch should be here tomorrow thank the gods.
How smart you were/are! Until all the sterility issues arose, I thought purity on the COA meant it was pure and sterile. And that you didn't with compound pharmacy product is amazing!
Since you don't like Amazon for sterile vials with self healing ports, can I ask where you are buying your vials? I just checked Amsco Med and westend and Farris and don't see vials or not in the small sizes I want.
Yea I wonder what the sterility of compounded ones are. They must be getting the powders from the same sources everyone here is using.
To get them sent to me in NY is nearly impossible but found a place and the price was high but I kept hearing they are the best! Nope.
Per Pep Test over on the Amo test channel on Discord, he's aware of instances when compound pharmacy products failed sterility. If they are all getting it from the same place, already lyophilized, if a sterility problem at that stage, reconning it here wouldn't change that.
moonpies4misfits
Advanced Researcher
I haven't purchased any yet, but there are a few med lab supply places that carry them. Having trouble finding 3ml, but here are some places I've looked:
Clear 2 ml - 5 ml - Sealed Sterile Vials - Glass Vials & Accessories - Lab Supplies | Med Lab Supply
Clear sealed glass vials (2ml - 5ml). MedLab has a large selection of sealed glass vials in various sizes, quantities and seal colors.
www.medical-and-lab-supplies.com
Empty Medical Sterile Glass Viles for Injection
Discover a range of sterile glass vials, perfect for injections and empty medication storage. Explore our selection today to meet your diverse medical needs.
westendmedicalsupply.com
There was a 3rd one that was all lab supplies but I can't find it right now
moonpies4misfits
Advanced Researcher
Damn, even through Diabetic/Medical supply websites? I can't order them off Amazon if I tried due to restrictions, but I've been happy with TDS so far.
Even through diabetic medical supply companies, not to NY without a prescription. Asked SlimdownRx where I got my compound initially for a prescription for BD syringes and he actually provided one to TDS. Asked for all sizes, he sent 3 prescriptions for the 3 sizes, TDS says they only received one for 1cc and I rarely use that size except when reconning.
This is my concern honestly. I started looking into filters after reading a lot of the sterility posts here and on chats. However I honestly don't trust random Amazon "sterile" products any more than I trust CN peptide vendors so am I really just adding the contamination risk by adding Amazon filters and empty vials into the mix? I'm starting to think for subQ research it's going to be fine just going for it.
I guess I used the wrong search terms on that site - you're so nice to have attached the link- interestingly the only way to get the vials from them shrink-wrapped is to order 25 or more.I haven't purchased any yet, but there are a few med lab supply places that carry them. Having trouble finding 3ml, but here are some places I've looked:
Clear 2 ml - 5 ml - Sealed Sterile Vials - Glass Vials & Accessories - Lab Supplies | Med Lab Supply
Clear sealed glass vials (2ml - 5ml). MedLab has a large selection of sealed glass vials in various sizes, quantities and seal colors.
Empty Medical Sterile Glass Viles for Injection
Discover a range of sterile glass vials, perfect for injections and empty medication storage. Explore our selection today to meet your diverse medical needs.
There was a 3rd one that was all lab supplies but I can't find it right now
moonpies4misfits
Advanced Researcher
Update: I was wrong, they weren't cheaper at Westend than TDS. I mistakenly ordered a 50 count thinking it was a box. Pricing is about the same as TDS.
I may continue to order from them as they also sell sterile vials and various Hospira solutions. The bac water is kinda pricey but it's nice to know I can conveniently get all my supplies in one place if needed. I'm good on bac for at least 6 months though.
Ok, Prepare for two dumb questions as they both popped into my head while reading this thread.
1st is: Doesnāt the BAC water, since it has alcohol in it to prevent bacteria, have properties to kill any minor sterility issues (assuming the vial doesnāt have obvious visible issues like mold or discoloration)?
2nd is: at 0.22 or 0.20 microns doesnāt the filter filter out the peptide as well? I mean that sounds super tiny and if it can filter out bacteria, what about short chain amino acids (aka peptides).
These were just the couple things that were in my head while reading so i appreciate any answers and dont mind snickering ;-)
1st is: Doesnāt the BAC water, since it has alcohol in it to prevent bacteria, have properties to kill any minor sterility issues (assuming the vial doesnāt have obvious visible issues like mold or discoloration)?
2nd is: at 0.22 or 0.20 microns doesnāt the filter filter out the peptide as well? I mean that sounds super tiny and if it can filter out bacteria, what about short chain amino acids (aka peptides).
These were just the couple things that were in my head while reading so i appreciate any answers and dont mind snickering ;-)
scarywood75
Scarlett Johansson
BAC water stops the growth of bacterias, but it does not kill the ones already present in the solution.
Most peptides are 0,001 to 0,005 micron in size, so a 0,22 micron filter will not remove them out.
Not sure if someone else had posted this and I grabbed it...OR I found it while searching myself.
My mind isn't what it used to be. š
These are directly from Cobetter
0.22Ī¼m PES Syringe Filters for Sterile Filtration, Sterile Individually Packed
I am looking at the double filter 13mm ones that have a .65 prefilter + .22um main filter, sterile and individually packed.
As of right now they are on sale for $41.30 for a packet of 100. Normal cost is $59.00
Looks like shipping may be as much as $27.50 so total is $68.80
They really need to fix that shipping cost! But still less than $ .70 each.
Hadn't seen these anywhere else, Let me know if you can find the same ones lower. Really like the double filter for the same price. I had already ordered some other ones from my earlier posts but I think these will be the next ones I get. Still wish they would make them in a 4mm package...maybe I will send them an email at some point
My mind isn't what it used to be. š
These are directly from Cobetter
0.22Ī¼m PES Syringe Filters for Sterile Filtration, Sterile Individually Packed
I am looking at the double filter 13mm ones that have a .65 prefilter + .22um main filter, sterile and individually packed.
As of right now they are on sale for $41.30 for a packet of 100. Normal cost is $59.00
Looks like shipping may be as much as $27.50 so total is $68.80
They really need to fix that shipping cost! But still less than $ .70 each.
Hadn't seen these anywhere else, Let me know if you can find the same ones lower. Really like the double filter for the same price. I had already ordered some other ones from my earlier posts but I think these will be the next ones I get. Still wish they would make them in a 4mm package...maybe I will send them an email at some point
Have you used the 13mm ones yet? how much have you noticed you lose in a 2ml vial? Thats my only concern with the 13mm ones is how much do you lose. Iād need to adjust how much BAC i add to offset the loss from the filter.
Not yet. I ordered the 13mm Biomed ones I linked earlier and they are scheduled to arrive Tuesday.
My plan is to fill a syringe with 1 or 2 ml of BAC water then put it through the filter and measure what I get out so I know the filter loss.
My plan is...Was I know this answer I will filter my peptide through the filter...then add straight BAC water in that amount into my syringe and "flush" the filter into the filtered vile to get most of the peptide out of it...
That is the CURRENT plan but I am open to suggestions. I will update here once I have the answers. Hopefully by late next week. š
scarywood75
Scarlett Johansson
A negligible amount as per the table on the page : 0,025ml. Can be diminished if you push air through it.
BD's are the effing worst. So glad to see someone else thinks so. I returned an entire box I bought. Bent pins, bend when injecting, trash. I much prefer Easy Touch
Chizikitide-1977
Research Enthusiast
That was me, still haven't used them though. Seems like everyone is shoping for and researching filters but hardly anyone is actually doing it yet.
The same filters are on Amazon in 10 packs, but the price per filter is higher. For most medical supplies, Amazon isn't my choice for this type of thing, but personally I'd be comfortable buying from the official Cobetter store on Amazon.
Thanks I just couldn't find the original post at the time.
When/If I get around to getting some of these I will be ordering the double filter versions I think.
I wasn't able to find the "double" filtered ones on Amazon at all just the single filter ones....If you have it would you mind posting.
On the Cobetter website you can select either the double 0.65+0.22um filter or the single 0.22um filter. Both at the same price.
My understanding from reading is that the "double" has a "pre" filter that is 0.65um followed by a 0.22um filter in the same package.
Chizikitide-1977
Research Enthusiast
Huh, I didn't realize that. I think I got the single filter version, but I would have gone for the double if I had read a bit closer. But then again I think that would increase losses. And yeah, looks like Amazon only has the single.
Edit: I did get the doubles! I'll report back on loss once I test them.
Chizikitide-1977
Research Enthusiast
Ok NOW I feel a little stupid...I was so busy looking for the double filter option on Amazon to match the Cobetter website choices, I missed the first bullet point under the pricing/quanity.
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- š[Unique Structure]The double-layer asymmetric PES hydrophilic membranes behave graduated pore size structure, a larger size of prefilter and a 0.22Ī¼m sterilizing-grade final filter
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scarywood75
Scarlett Johansson
I've ordered some directly from Cobetter, we shall see how it goes.
bradyfan127
New Member
Here's a link to some .22um 4mm filters. 200 pack for $100... Not sure if any of you are familiar with the brand or have previously tried.
filtrous.com
PTFE Single Layer (DX) Non-Sterile, Syringe Filter, 0.22um, 4mm Diameter, 200/unit
Shop Filtrous PTFE Single Layer Syringe Filter, 0.22Āµm, 4mm, featuring a dual-layered PTFE membrane for enhanced filtration efficiency. Compatible with nearly all solvents and designed to handle high backpressure. DNase/RNase-free and non-pyrogenic for reliable, contamination-free sample...
filtrous.com
Chizikitide-1977
Research Enthusiast
Also doesn't say if they're sterile.
I ordered the single layer from Cobetter (13mm, tiny!) for some sus cagri I have, they just came today. I used water, an empty vial, a luer lock syringe/needle, and one of the filters to practice with.
2ml water into the vial, pre-wet the filter, pulled the water out with syringe (made sure I drew it all out), removed needle, attached filter, reattached needle, pushed the water back into the vial.
There was resistance I didnāt expect (I also created a vacuum in the vial somehow, no clue), so it went slower than I expected. Removed the syringe part to do that āflush,ā pulled air in, reattached, pushed through to flush the filter.
Finally, I pulled the contraption apart, and pulled up all of the water to see how much Iād lost. Virtually none. So little Iām going to have to do this a few more times to make sure.
Pic of the little filter
2ml water into the vial, pre-wet the filter, pulled the water out with syringe (made sure I drew it all out), removed needle, attached filter, reattached needle, pushed the water back into the vial.
There was resistance I didnāt expect (I also created a vacuum in the vial somehow, no clue), so it went slower than I expected. Removed the syringe part to do that āflush,ā pulled air in, reattached, pushed through to flush the filter.
Finally, I pulled the contraption apart, and pulled up all of the water to see how much Iād lost. Virtually none. So little Iām going to have to do this a few more times to make sure.
Pic of the little filter
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Fonkymonky
Established Member
In the meantime I found the right filters (I hope š
) but apparently instead of the 13mm diameter I bought the 25mm diameter (see pictures) I hope this will not cause any problems? Can someone tell me the dimensions of the syringe and needle I have to get to match these? Also, is there a website appart from Amazon where I can find the sterile vials?
Thanks in advance!
Thanks in advance!
Attachments
moonpies4misfits
Advanced Researcher
Just make sure you get luer lock syringes. The needles will fit both luer lock and luer slip, 25g-27g are what I see a lot of people using
noteablequotable
Advanced Researcher
Please let us know how this goes for you. Id definitely prefer this to standard insulin needles.
Fonkymonky
Established Member
Thanks! š
noteablequotable
Advanced Researcher
If you're worried about lost product the Peptide Test YouTube channel has a good video on this. Makes sense that you lost very little water.
I don't think that material is recommended for peptides. I think PES is what you want.Here's a link to some .22um 4mm filters. 200 pack for $100... Not sure if any of you are familiar with the brand or have previously tried.
PTFE Single Layer (DX) Non-Sterile, Syringe Filter, 0.22um, 4mm Diameter, 200/unit
Shop Filtrous PTFE Single Layer Syringe Filter, 0.22Āµm, 4mm, featuring a dual-layered PTFE membrane for enhanced filtration efficiency. Compatible with nearly all solvents and designed to handle high backpressure. DNase/RNase-free and non-pyrogenic for reliable, contamination-free sample...
Not so much worried as surprised at how little I lost. Folks are really trying to hunt down the 4mm, but I think the 13mm appear to do just fine.
Fonkymonky
Established Member
I ordered the 25mm. Do you think it makes a big difference?
Someone please correct me if I'm wrong, but my understanding is that .25mm doesn't work well at all for the small volumes we're dealing with when reconstituting peptides. The amount that stays behind in the filter is something close to a ml.
It will, but still maybe not as much to make a difference to you, though Iām not you. Looking at the cobetter site, it says the āhold-up volumeā is less than 25 microliters for the 13mm, less than 100 microliters for the 25mm.
So I think youāll notice a little bit, but weāre talking microliters. How much does that little bit matter to you? And those numbers are pre-purge, I think. (Donāt quote me, I havenāt worked in a lab since 2012 and even then I wouldnāt have quoted me.)
and piggybacking off of the last person, the site says the 25mm are best for samples 10ml to 100ml, so idk
Iām looking at couple other sites and they say their 25mm can be used for as little as 2ml, with a hold up volume of less than 50 to as much as 115 microliters, so maybe itās brand specific?
bradyfan127
New Member
Thanks for letting me know. I've been going back and forth on this. Chatting on another board and reading information on here has actually helped. I've only been here one day and the conversation is WAY WAY more productive.
