In Novo’s Own Words: Degradation of Amylin Analogs Such as Cagrilintide (and How to Test For It)

[Dee X]

lmao. first off you didn't even read OP's opening argument as you were caught stupidly spamming his own citation back at him thinking that his argument hinged on fibrils and now after going dark for a few days, you're now spamming "gotchas" as if you've solved something?

ol tessa here has posted one comment with her argument plus citations and you're out here running victory laps. settle down, turbo. i'm sure OP will be back to respond in kind and the conversation can continue on.

NurseRatchit's got the fibril fever.

Tessa posted this in multiple places. There are conversations going on in tandem. This isn't the first time fibrils + reconstitution pH were discussed and heavily debated in peptide communities since this info came out 3+ years ago and Cagri early this year.

 

[DevynL90]

NurseRatchit's got the fibril fever.

Tessa posted this in multiple places. There are conversations going on in tandem. This isn't the first time fibrils + reconstitution pH were discussed and heavily debated in peptide communities since this info came out 3+ years ago and Cagri early this year.

i'm aware, i've seen the argument play out in the peptidetest.com discord and i'm not the only one that left that conversation feeling convinced in secretweapon/megalith's over tessas argument that cagri is likely to be dangerous.

no one else was taking victory laps in this thread, it's incredibly weird and awkward to see nurseratchit attempting to do such, lol.

 

[nurseratchit]

lmao. first off you didn't even read OP's opening argument as you were caught stupidly spamming his own citation back at him thinking that his argument hinged on fibrils and now after going dark for a few days, you're now spamming "gotchas" as if you've solved something?

ol tessa here has posted one comment with her argument plus citations and you're out here running victory laps. settle down, turbo. i'm sure OP will be back to respond in kind and the conversation can continue on.

I wasn't 'caught' I simply glazed over his wall of text because this 'argument' was rehashed ages ago elsewhere.

If by, going dark, you mean going to my job and living a productive life outside of arguing with internet trolls they, yes I am guilty.

Obviously, SW, is your hero and your reliable source so I will leave him all to you fanboi.

 

[DevynL90]

I wasn't 'caught' I simply glazed over his wall of text because this 'argument' was rehashed ages ago elsewhere.

If by, going dark, you mean going to my job and living a productive life outside of arguing with internet trolls they, yes I am guilty.

Obviously, SW, is your hero and your reliable source so I will leave him all to you fanboi.

ahhhh, yes, you're the unique one here with the job. no one else has a job with other responsibilities, just you. yet somehow you managed to reply back to the thread within what, 60 minutes of tessa posting? total coincidence i'm sure.

just stop being weird about this already. no one's won anything. stop pretending like any side has been proven correct.

 

[Dee X]

i'm aware, i've seen the argument play out in the peptidetest.com discord and i'm not the only one that left that conversation feeling convinced in secretweapon/megalith's over tessas argument that cagri is likely to be dangerous.

no one else was taking victory laps in this thread, it's incredibly weird and awkward to see nurseratchit attempting to do such, lol.

This was heavily debated way before peptest existed, no one took victory laps then either since people weren't in it for ego via "I'm more right than you, even if I'm wrong" as much as they are now.

NurseRatchit has been hit by the oligomer syndrome, it's what you've been warning all of us of. She's an oligonner now.

 

[peptideusername]

She's an oligonner now.

 

[exploitedworkerbee]

I wonder if cagri is more or less risky than those mail order bath salts I got into for a spell during grad school. If that stuff didn’t get me I can’t believe this stuff will.

 

[clayd]

NurseRatchit's got the fibril fever.

Tessa posted this in multiple places. There are conversations going on in tandem. This isn't the first time fibrils + reconstitution pH were discussed and heavily debated in peptide communities since this info came out 3+ years ago and Cagri early this year.

but still nobody seems to have the answer. surprised there's not one or 2 people out there with some access to testing equipment or a microscope or whatever who can say "looky what i found"

i mean i know this is specialized info but the internet always seems to find some highly trained individual who can help clear things up

 

[Ladylaw]

 

[49metal]

OK. If I were to credit this warning I would need to target a pH of 4. I already reconstitute Cagri using Hospira Bacteriostatic 0.9% Sodium Chloride which has a pH 5.0. The resulting reconstituted Cagri has a pH of 5.0-5.25. I have Acetic Acid 0.6% Solution with a pH of about 3.0. I'm confident I can use it knock the pH of my reconstituted Cagri down to 4.0.

Now, assuming I do this, does 4.0 really address the issue? Or will I be made to understand there are yet more reasons I can't shoot my Cagri?

 

[zpped]

OK. If I were to credit this warning I would need to target a pH of 4. I already reconstitute Cagri using Hospira Bacteriostatic 0.9% Sodium Chloride which has a pH 5.0. The resulting reconstituted Cagri has a pH of 5.0-5.25. I have Acetic Acid 0.6% Solution with a pH of about 3.0. I'm confident I can use it knock the pH of my reconstituted Cagri down to 4.0.

Now, assuming I do this, does 4.0 really address the issue? Or will I be made to understand there are yet more reasons I can't shoot my Cagri?

If you believe in the warning, it's already too late to change the ph. The ph would have been wrong even before it was lyophilized.

 

[hexagonal]

In theory it should help prevent additional fibril (and oligomer? maybe?) formation though, so it wouldn't be the worst idea.

I am not an expert and this whole subject made me cancel my prior plan to order some cagri to stack with my reta, but if I was going to go through with it, this is what I would do:

1) Use smaller dosage vials so I go through them more quickly
2) Reconstitute and get the pH to 4.0
3) Filter after to remove as many of the larger clumped fibrils as I can

From some posts in the other forums, it looks like NN is now testing some single chamber pens with combined CagriSema, though we don't yet seem to have details on the pH, buffering agent used, etc.

 

[peptideusername]

oligomer?

 

[exploitedworkerbee]

View attachment 4210

This will never be played out. Please never ever ever stop.

 

[JohnnyGobbs]

Anyone have a recommendation for ph test strips you guys use for testing ph, preferably something on Amazon. Thx

 

[zpped]

Anyone have a recommendation for ph test strips you guys use for testing ph, preferably something on Amazon. Thx

It's hard to go wrong with ph paper. It's an extremely basic thing all over amazon.

 

[kussmaul]

To summarize it from that thread, it is obvious the centrifuge test was meant to be a proxy for potential long term degradation - that was the whole point. A 45 hour test is not useful for any sort of commercial applications otherwise, since no one prescribed cagrilintide would be taking it within 45 hours of formulation, or using it in a centrifuge at high heat. It is an attempt to recreate the sort of results they expect cagrilintide might have when reconstituted against the long term. And, ultimately, they could have gone with that formulation at a pH of 7.5 and not had to develop a whole new pen that kept cagrilintide and semaglutide separate, significantly increasing their cost, but instead decided that doing so was necessary.

Not that it really matters to these argument, but the testing referenced was not done using a centrifuge. A fluorescence plate reader was used. The plate reader moved the plate in a 1 mm orbit 960 rpm, so small fast circles. Centrifuge speeds are much faster with more movement. Centrifuges are meant to separate whatever is being spun into components, the plate reader is meant to keep the solution mixed (usually keep the fluorescence from settling).

 

[49metal]

extremely basic

 

[YoYoFat]

It's hard to go wrong with ph paper. It's an extremely basic thing all over amazon.

Sincere question from a lay person.

I think I understand that a big question surrounds pH and stability of pH in its relation to the very bad things that might form and turn your pancreas and brain into mush.

And I get that the pep might have a pH of X upon reconstitution, but doesn’t the pH of the stuff you inject into your body change to Y upon mixing with “body stuff”?

 

[hexagonal]

Not that it really matters to these argument, but the testing referenced was not done using a centrifuge. A fluorescence plate reader was used. The plate reader moved the plate in a 1 mm orbit 960 rpm, so small fast circles. Centrifuge speeds are much faster with more movement. Centrifuges are meant to separate whatever is being spun into components, the plate reader is meant to keep the solution mixed (usually keep the fluorescence from settling).

Clarification is always good. Someone said centrifuge at some point and I just started copying it.

I think it does matter, though, since it sounds like this is significantly less of a torture test than it has been made out to be.