Putting Air in the syringe and into your vial.

[Whiynot20026]

To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill

 

[dgoldrich]

I put air in my vials every time...never had an issue

 

[quicksilver80]

Some of these vials have such a hard vacuum id lose weight from waiting to draw a dose and otherwise unable to use my hands for anything else. Be old and grey by the time that shits done with a 31g needle lol

 

[tcpnomad]

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

 

[Whiynot20026]

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

I have no issues at all. The 31s are slow. The 30s are better. It drips in like an IV. But I’m doing super small doses currently. Nothing over 3mg. I could easily see doing a large dose, and adding air as a benefit for sure.

Bill

 

[bada bing]

That's the purpose of the benzyl alcohol in the BAC. It stops any bacteria growth for a reasonable duration of usage after reconstitution.

I usually inject the same amount of air as I'm intending to pull out.

 

[BNLFL]

I put air in my vials every time...never had an issue

Same here.

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

Not all do, and not when you filter into a new vial. I normally vent, so no pressure at all. Pushing the air in lets you draw the product into the injection syringe. Drawing into the vials with a 31g is slow enough.

 

[tcpnomad]

I normally vent, so no pressure at all.

Well, that's putting air into the vial 😛.

 

[eidos]

To be or not to be. I read things every day that putting air in the syringe can contaminate the vial. I personally don’t do it. But I have a few friends that do. My philosophy is the more sterile the protocols used the better off your peps will be.

Bill

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

 

[mybodyisasewer]

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

Posts like this are why I'm here but also make me wonder how I am not dead.

I don't know how much of that is realistic for me to implement, but I recently started using caps for the reconned vials and I wipe those out with an alcohol swab. I like the mask idea I will probably start doing that.

Now I need to look up vent needles.

 

[Whiynot20026]

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

Wow,

You do what I see in some YouTube videos. That’s great. I do alot of things you recommend. So far it has been a very sterile environment in my home.

Bill

 

[Mochizuki0124]

For my first few pulls I usually add air but around my 3rd or 4th pull usually its no longer necessary.

So basically I do both.

 

[sheilarae74]

I do it and never had any problems. Maybe if I was immune compromised I wouldn't.

 

[5byfive]

Yes, it can.

Mainly Pseudomonas bacteria in the air (against which benzyl alcohol has little effect). Staph that shed from your skin, body hair/hair. Possibly a few fungal spores from you and your clothes. Above all, the entire microbiome surrounding you within a radius of about one meter, which you release with every exhalation. Enough? ;-)

That’s why you need to thoroughly disinfect your hands or wear alcohol-treated gloves, remove watches and jewelry, wear a mask (I still have plenty from the COVID...), and work in a clean, windless environment (nowadays, I have to turn off the AC and fans, and with it being 40°C outside, that’s tough!).

Watch nursing training videos to learn best practices.
Definitely avoid random YouTube videos (especially PepCritic; I just watched one, and it made my skin crawl how badly he does it!).
I prepare the recons in batches in pen vials. The venting problems you have don't occur when injecting with a pen. It's really a consequence of the multi-dose in a vial.
I put a hairnet on my head and a non-woven lab coat during recons (not during injections). They're really inexpensive, and you can use them for a while: when you're working alone, there's no risk of cross-contamination. Just, watch out for the cat!

You can use a filter attached to the end of a vent needle to equalize the pressure. This filter can be reused for a while if you store it properly. PTFE is good for air, but PES is okay too. 0.1µm PTFE if you’re really paranoid ;-) Even paranoids can have enemies: Pseudomonas and Staphylococcus are nasty.

99% of people aren't doing that and I'm not seeing reports of issues becuse of it. I am all for mitigating risk but that seems a bit much. Maybe for someone who immunocompromised it would make sense but otherwise it is a bit much for my taste.

I have a friend who gets 200% into everything he does (unlike me who tries to keep it more like 150%) and I always say his motto is "If its worth doing, its worth overdoing". Sounds like something he would do.

 

[Flash-BCR]

I put air in...I try and be a tidy as I can with recon, but my main concern is a vial clouding before 6-8 weeks. Not really concerned about getting sick...

 

[Sundaycandy]

I’m team air in the vial. It’s just too difficult not to in my opinion. Maybe I could try being more patient lol

 

[dolph]

A couple times I've taken a fresh syringe and pulled vacuum again.
No idea if it helps but it made me feel better.

I try not to add air but pulling 75 units of MOTSc without adding a little seems almost impossible w/ a 31g.

 

[Whiynot20026]

I’m team air in the vial. It’s just too difficult not to in my opinion. Maybe I could try being more patient lol

I meet fellow lab rats that tell me they pin sometimes 10-15 times. They should hire a nurse to help out. Which is another discussion on pens or syringes. I love syringes for so many reasons. But so many just explode on telling me to go pen. We will see. One day maybe. There’s just something about sticking a 5/16 needle into my belly. Like a punishment for being so unhealthy the last few years. When someone offers me something unhealthy I mentally image the needle going into my belly, and I politely say no thank you.

Bill

 

[HARLEYV2]

99% of people aren't doing that and I'm not seeing reports of issues becuse of it. I am all for mitigating risk but that seems a bit much. Maybe for someone who immunocompromised it would make sense but otherwise it is a bit much for my taste.

I have a friend who gets 200% into everything he does (unlike me who tries to keep it more like 150%) and I always say his motto is "If its worth doing, its worth overdoing". Sounds like something he would do.

Ask people who have taken insulin every day of their life how they do it lol ?

 

[Tirzmonkey]

Try drawing the last dose out of a 60mg vial without injecting air. Good luck with that.

 

[BNLFL]

Well, that's putting air into the vial 😛.

What the hell are you talking about? Please explain your logic, I'd love to hear it. Do you filter your stuff? You vent the new vial to help with pushing the reconstituted product into the new vial. All of the new vials I've used have no vacuum, so pushing 2-3ml of reconned product, the syringe will fight back.

 

[eidos]

I have a friend who gets 200% into everything he does (unlike me who tries to keep it more like 150%) and I always say his motto is "If its worth doing, its worth overdoing". Sounds like something he would do.

Overdoing would be to do the same thing as in hospital pharmacies when injectable solutions are prepared. Your friend might want to buy this 🤣
https://www.erea.fr/isolateur-sterile/

 

[bada bing]

Ask people who have taken insulin every day of their life how they do it lol ?

Lol, 100%
My ex-wife is a T1D. Been doing multiple SubQ injections per day for over 5 decades. About 3+ decades of that was with standard syringes from multi-does vials. New vial, air goes in, dose is metered out. Watched hundreds of times.

Here's something that will really gross out germ-phobes. Guess how often on average a T1D change out the needles on their pens ? If you guessed once per day on average, with several shots per needle you'd get the prize. Somedays they forget to change the needle. That's 10's of thousands of people doing that, everyday.

 

[AlexSilver]

Lol, 100%
My ex-wife is a T1D. Been doing multiple SubQ injections per day for over 5 decades. About 3+ decades of that was with standard syringes from multi-does vials. New vial, air goes in, dose is metered out. Watched hundreds of times.

Here's something that will really gross out germ-phobes. Guess how often on average a T1D change out the needles on their pens ? If you guessed once per day on average, with several shots per needle you'd get the prize. Somedays they forget to change the needle. That's 10's of thousands of people doing that, everyday.

Insane. Not counting the sterility issue, plunging any needle into me a second time just kills me. Super painful. Pen needles too.

 

[bada bing]

If you want to see how T1D deal with their meds, there are all kinds of videos on Youtube sharing advice. Some of it is applicable to peptide pinners as well.

I would never ever reuse a needle. But T1D get pretty cavalier about the whole proces after 1/2 a lifetime. As a Tirz user for 19 months now, I just ran out of my first box of 100 syringes.

View: https://www.youtube.com/shorts/2WGi8KNpnFM

 

[AlexSilver]

If you want to see how T1D deal with their meds, there are all kinds of videos on Youtube sharing advice. Some of it is applicable to peptide pinners as well.

I would never ever reuse a needle. But T1D get pretty cavalier about the whole proces after 1/2 a lifetime. As a Tirz user for 19 months now, I just ran out of my first box of 100 syringes.

Maybe after half a life or more you get numb to it but after 8 months, I’m tender!

 

[HARLEYV2]

Lol, 100%
My ex-wife is a T1D. Been doing multiple SubQ injections per day for over 5 decades. About 3+ decades of that was with standard syringes from multi-does vials. New vial, air goes in, dose is metered out. Watched hundreds of times.

Here's something that will really gross out germ-phobes. Guess how often on average a T1D change out the needles on their pens ? If you guessed once per day on average, with several shots per needle you'd get the prize. Somedays they forget to change the needle. That's 10's of thousands of people doing that, everyday.

Was my point exactly. Been a CC Paramedic for years. The only Med I filtered was Amiodarone. We use a needle straw filter for that.

 

[BigDog8084]

I wonder how it even works for you then?! I mean, the vials have a vacuum in them. Is it hard to get the peps out?

Good question, I cant draw without putting some air in there. We also have very good air quality and I run an air purifier (with hepa filter) in the house.

 

[HARLEYV2]

Lol, 100%
My ex-wife is a T1D. Been doing multiple SubQ injections per day for over 5 decades. About 3+ decades of that was with standard syringes from multi-does vials. New vial, air goes in, dose is metered out. Watched hundreds of times.

Here's something that will really gross out germ-phobes. Guess how often on average a T1D change out the needles on their pens ? If you guessed once per day on average, with several shots per needle you'd get the prize. Somedays they forget to change the needle. That's 10's of thousands of people doing that, everyday.

I will start Filtering My Peps though.

 

[bada bing]

I will start Filtering My Peps though.

Yes. Me too.
I have a couple convi pens on the way, as well as some filters, cartridges and Luer lock 3mls. I'm taking my first foray into pens. I'm going to add Reta and KPV into the weekly rotation for a while and I'm curious about pens and multiple pokes per week instead of my once a week ritual..