Hydrophilic PVDF also has an affinity for low-protein binding. PVDF is typically hydrophobic so make sure the filter is treated so it’s hydrophilic.
Filters of the same diameter/material from different manufacturers may have different holdback volumes. The key, from my experience, is to purge with bac water and air so the concentration of the filtered peptide remains unchanged.
Filters of the same diameter/material from different manufacturers may have different holdback volumes. The key, from my experience, is to purge with bac water and air so the concentration of the filtered peptide remains unchanged.