Syringe Filter

Ok thanks for this! One more question. You think I could add BAC water to the vials I am already using? Would that make any difference? Or should I best toss the vials in the bin?
BAC water does not kill the bacterias, it just stops them from multiplying while they are in the presence of benzyl.

So when you inject to your RS, the bacterias in the solution will start to multiple again.

You can't use that for making a solution sterile if that's your question
 
BAC water does not kill the bacterias, it just stops them from multiplying while they are in the presence of benzyl.

So when you inject to your RS, the bacterias in the solution will start to multiple again.

You can't use that for making a solution sterile if that's your question
Ok good to know. Thanks for the info!
 
Would injecting the Bac water into his vials and then filtering it into another sterile vial work?
Interesting question! Logically, that seems reasonable but I wonder how that would pan out in practice.
But also you’d have to reach an effective concentration of benzyl alcohol which will be hard to do unless you’re gonna massively dilute with a bunch of bac water.
 
Yeah true, I was referring to the concentration present in BAC water, of course injecting yourself with any higher concentration can be bad like everything else
You were very clear in your initial explanation. And the bottle is marked not for neonates.
 
What else does the bottle say?
Here’s the page from the nih where they have about 4 diff approved labels you may see. What determines that, idk, market, manufacturer?

The ones I have are the top left label.
 
Has anyone noticed any decreases in efficacy, or any negative side effects since they began using filters?
 
Have you noticed any? My stuff gets delivered today, but I still have another 7 weeks of compound.
 
So I finally got around to trying out my Co better .65/.22 um filters. I filtered 3 milliliters of bacteriostatic water and lost a total of .5 milliliters. However this loss is likely on the high side due to my inexperience. Next time I’ll have to make sure that the container I filter into has a vacuum. A small amount of liquid was lost because the pressure difference between the syringe and the vial led to that liquid shooting back into the syringe at the last second and then out the syringe.

I think with a better technique next time I could probably cut this down to around .3 or .4 milliliters.

I also tested out my HUMAPEN Lily ergo II pen. This was the cheapest pen you can find off of aliexpress that comes with a case. $15 at the link below and that includes free shipping.

https://www.aliexpress.us/item/3256806314136649.html

The pen is compatible with standard insulin cartridges and pen needles. It seems simple enough to use and has the same setup as the pens sold on pep pens or other resellers. It’s not spring loaded and has a dial that can deliver up to 60 units. Definitely a much better deal than buying a similar pen off of one of the resellers.
 
So I finally got around to trying out my Co better .65/.22 um filters. I filtered 3 milliliters of bacteriostatic water and lost a total of .5 milliliters. However this loss is likely on the high side due to my inexperience. Next time I’ll have to make sure that the container I filter into has a vacuum. A small amount of liquid was lost because the pressure difference between the syringe and the vial led to that liquid shooting back into the syringe at the last second and then out the syringe.

I think with a better technique next time I could probably cut this down to around .3 or .4 milliliters.

I also tested out my HUMAPEN Lily ergo II pen. This was the cheapest pen you can find off of aliexpress that comes with a case. $15 at the link below and that includes free shipping.

https://www.aliexpress.us/item/3256806314136649.html

The pen is compatible with standard insulin cartridges and pen needles. It seems simple enough to use and has the same setup as the pens sold on pep pens or other resellers. It’s not spring loaded and has a dial that can deliver up to 60 units. Definitely a much better deal than buying a similar pen off of one of the resellers.
4mm filters steal 7 units and will work for filtering up to 4ml.

13mm filters steal 28 units and are good for up to 8ml - I think it is.

I don't see any reason to use a filter larger than 13mm.

You can also pre-wet the filter with bac, and air purge after filtering to help reclaim a bit of what they steal/absorb. Just don't blast anything through with high pressure as it can rupture the filter. Slow and steady. If it fails, start over with a new filter and don't press so hard, lol

PeptideTest has some YT videos about this.
 
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So I finally got around to trying out my Co better .65/.22 um filters. I filtered 3 milliliters of bacteriostatic water and lost a total of .5 milliliters. However this loss is likely on the high side due to my inexperience. Next time I’ll have to make sure that the container I filter into has a vacuum. A small amount of liquid was lost because the pressure difference between the syringe and the vial led to that liquid shooting back into the syringe at the last second and then out the syringe.

I think with a better technique next time I could probably cut this down to around .3 or .4 milliliters.

I also tested out my HUMAPEN Lily ergo II pen. This was the cheapest pen you can find off of aliexpress that comes with a case. $15 at the link below and that includes free shipping.

https://www.aliexpress.us/item/3256806314136649.html

The pen is compatible with standard insulin cartridges and pen needles. It seems simple enough to use and has the same setup as the pens sold on pep pens or other resellers. It’s not spring loaded and has a dial that can deliver up to 60 units. Definitely a much better deal than buying a similar pen off of one of the resellers.
Glad to hear that the Ergo 2 seems to be working out. I have one coming in in the next week or so.
 
PeptideTest has some YT videos about this.
Yes, I've posted links to those videos on yt and seen his numbers. However I've become a bit skeptical of some of it the more I dig.


First his numbers for loss should be proportional to the surface area of the filter, but his numbers are proportional to the diameters. This makes no sense physically. I asked him about this on peppys but he never replied. There are also differences between brands for retention volume, and he doesn't mention that.


Also fetefille posted lower loss numbers earlier in this thread, but for the single filters from Cobetter. I would expect higher losses from the doubles I'm using but neither match the numbers peptidetest posted.


Also, I saw peptide test write on one of the boards (dont remember which) that he's discourages using the "cheap" Cobetter filters. Which is fine, but he is the only person I've seen say these are "cheap", literally every other thing ive read said thse are high quality. And I DUG before I bought these.


Between that and him advocating for using 4mm, he is effectively advocating for using a type of filter that is nearly impossible to find. It just so happens he is the only place that sells them without buying 100+. One of the mods on peppys called him out for this a couple days ago.


Even if we ignore this conflict of interest I generally get the sense peptide test is kind of learning on the go with filters. That's fine, and the info is still helpful. I just don't take what he says as the final authority on this matter.
 
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Yes, I've posted links to those videos on yt and seen his numbers. However I've become a bit skeptical of some of it the more I dig.


First his numbers for loss should be proptioal to the surface area of the filter, but his numbers are proportional to the diameters. This makes no sense physically. I asked him about this on peppys but he never replied. There are also differences between brands for retention volume, and he doesn't mention that.


Also fetefille posted lower loss numbers earlier in this thread, but for the single filters from Cobetter. I would expect higher losses from the doubles I'm using but neither match the numbers peptidetest posted.


Also, I saw peptide test write on one of the boards (dont remember which) that he's discourages using the "cheap" Cobetter filters. Which is fine, but he is the only person I've seen say these are "cheap", literally every other thing ive read said thse are high quality. And I DUG before I bought these.


Between that and him advocating for using 4mm, he is effectively advocating for using a type of filter that is nearly impossible to find. It just so happens he is the only place that sells them without buying 100+. One of the mods on peppys called him out for this a couple days ago.


Even if we ignore this conflict of interest I generally get the sense peptide test is kind of learning on the go with filters. That's fine, and the info is still helpful. I just don't take what he says as the final authority on this matter.
I had the same feeling about him.. learning on video for someone who runs a testing lab is a bit sketchy. He claims they tested the volume loss though and said they will post that vid soon. I'm sure it varies across filters. I've not seen anything else to go on though.

I'll be testing the 4mm soon. Seems like the best option for my needs.

I've had a hard time finding filters at all. Cobetter are 2 weeks out on my Amazon order. And like you said, direct from factory require buying 100-200 at a time.

I just don't like buying stuff like this on Amazon, lol All the med supply places I checked just have the non sterile filters in a bucket, not the ind sealed ones.

Options are limited
 
Part of me really wants to trust the guy implicitly since we NEED more options for testing, but in the peptide space that would be foolish. Jano is good and all but its not great that we all rely on 1 lab in the Czech rebublic for everything.

For this filtering buisness I think the best thing we can do is continue to experiment, compare notes and numbers and help each other dial in the technique.
 
Part of me really wants to trust the guy implicitly since we NEED more options for testing, but in the peptide space that would be foolish. Jano is good and all but its not great that we all rely on 1 lab in the Czech rebublic for everything.

For this filtering buisness I think the best thing we can do is continue to experiment, compare notes and numbers and help each other dial in the technique.
Is Jano generally regarded higher that PeptideTest for testing? I was thinking about using PT for my L60.
 
Is Jano generally regarded higher that PeptideTest for testing? I was thinking about using PT for my L60.
I've only recently entered the testing space, and am not the best authority on this, but generally yes Jano is considered the GOLD STANDARD from what I've read.

As previously stated, peptide test seems to be learning as he goes. Multiple people have made similar observations, but that doesn't mean you should automatically go with jano. Some people actually made a point of using peptidetest in part to help him get more experience and exposure so we have more options.
 
Is Jano generally regarded higher that PeptideTest for testing? I was thinking about using PT for my L60.
Jano is good for fill & purity and considered the gold standard in the community. Some vendors will only reship/refund based on their tests. But there are other options that I believe are just as good... MZ biolabs in Arizona, and I forget the other atm.. my brain is done for the day, almost 1am here, lol

PeptideTest was involved with some sterility testing for the SPC fiasco and "seems" to offer a better sterility test... But after seeing those videos and the highly generic, low level website and ordering process they use, I kind of question it. Maybe they just cheaped out on the website but it's not a good look for what's supposedly "better". I dunno, like @Chizikitide-1977 said, we don't really have a lot of options... It's expensive and the testing for sterility is pretty new. Standards need to be developed, bacteria identified, and safe level limits need to be set by the community. I think everyone is waiting on peppys to come out with this info.

In the meantime, I'll do what I can and use what's available to mitigate the best I can. But there's still a long ways to go to determine what the risks are, what's the best way to test, best way to mitigate bacteria, etc. Its a bit of a mess.
 
4mm filters steal 7 units and will work for filtering up to 4ml.

13mm filters steal 28 units and are good for up to 8ml - I think it is.

I don't see any reason to use a filter larger than 13mm.

You can also pre-wet the filter with bac, and air purge after filtering to help reclaim a bit of what they steal/absorb. Just don't blast anything through with high pressure as it can rupture the filter. Slow and steady. If it fails, start over with a new filter and don't press so hard, lol

PeptideTest has some YT videos about this.
He only used one type of filter in his study, a good kind, his preferred kind, but only one type, Tisch. He considers Cobetter generic, maybe all lab folks who use syringe filters do. The amount lost changes with the brand, not at an appreciable level to me, ymmv.
 
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I had the same feeling about him.. learning on video for someone who runs a testing lab is a bit sketchy. He claims they tested the volume loss though and said they will post that vid soon. I'm sure it varies across filters. I've not seen anything else to go on though.

I'll be testing the 4mm soon. Seems like the best option for my needs.

I've had a hard time finding filters at all. Cobetter are 2 weeks out on my Amazon order. And like you said, direct from factory require buying 100-200 at a time.

I just don't like buying stuff like this on Amazon, lol All the med supply places I checked just have the non sterile filters in a bucket, not the ind sealed ones.

Options are limited
All you can really do is take my word for it, but I'm on his discord channel, which was originally created when we were trying to figure out the logistics of the original Amopure group test we did. The filtering thing came up organically because of discussions other people were having. It wasn't something he just started promoting out of nowhere. He also wasn't selling them at first either, and when he did put some up on his site, it wasn't many. I think he was more trying to make them accessible to some of us who were having trouble finding them. It also doesn't make any sense that he would advocate for one filter and sell only that filter, go through the trouble of making videos for that one filter when he'd make more money selling both imo. Its not like he's selling truckloads of filters. He seems like someone who is just trying to be a resource to the community while taking the opportunity to grow his business.
 
Where is a good place to buy filters? I tried searching meso because I know bodybuilders use them for a lot of their stuff but couldn't find anywhere. I've tried to buy from some legit lab sites but I've had my orders rejected because they only sell to businesses or scientific labs. I'm leery of Amazon and Aliexpress even if it says "sterile", although I know people use them.
 
Okay, quick update on my 2nd practice round. Still using the .65/.22 13mm Cobetter double filters.

Took my time and was super careful this round. Also tried an air purge. Filtered 1 ml of bac water. After the air purge, losses were neglectable. 2 units lost out of 100 filtered. Just to make sure I didn't damage the filter after the air purge I filtered a bit more water and there was no difference in the amount of resistance. So I don't think I damaged the filter with the air purge.

I'll continue to experiment and report back (and others please do the same), but based on this, it seems losses for the 13mm filters might be a bit overstated. At least for the Cobetter filters.

One important piece of advice, before doing an air purge, make sure the vial/cartridge you are filtering into has a vacuum. I created a vacuum in the vial I was using by removing air with an insilin syringe, pulling out 3ml of air. Trying to air purge without vaccum was how I lost .5ml of the 3ml filtered last time.
 
I bought straight from Tisch with no issues. I have 200 of them but that should last a long time.
 
I bought from Peptide Test as I was buying Bac water and wanted everything together. They are a bit pricey though with shipping. Should have ordered more Bac water and filters to get the free shipping.
 
Where is a good place to buy filters? I tried searching meso because I know bodybuilders use them for a lot of their stuff but couldn't find anywhere. I've tried to buy from some legit lab sites but I've had my orders rejected because they only sell to businesses or scientific labs. I'm leery of Amazon and Aliexpress even if it says "sterile", although I know people use them.
The person who runs Peptide Test sells and recommends Tisch. I purchased mine from Cobetter and I really like them. Most will recommend 4mm, mine are 13mm. You will lose less product the smaller they are, but each brand holds back a different amount/volume, some more, some less, for the same sizes.
 
@fetefille, have you done any further tests on your Cobetter filters? You mentioned in your earlier post you thought there was a chance you did something wrong and your zero loss might be too good to be true.

I've now observed zero loss with the Cobetter filters after an air purge, but I'm also not 100% sure I did everything right. Just looking to compare notes.
 
One important piece of advice, before doing an air purge, make sure the vial/cartridge you are filtering into has a vacuum. I created a vacuum in the vial I was using by removing air with an insilin syringe, pulling out 3ml of air. Trying to air purge without vaccum was how I lost .5ml of the 3ml filtered last time.
I was planning on piercing my cartridges with a second sterile needle while I injected the solution to give the air somewhere to escape. Your solution makes a lot of sense, though. Did you have any trouble drawing that much of a vacuum?
 
I was planning on piercing my cartridges with a second sterile needle while I injected the solution to give the air somewhere to escape. Your solution makes a lot of sense, though. Did you have any trouble drawing that much of a vacuum?
That is what I do. It releases the vacuum and equalizes the pressure while you fill it.
 
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I was planning on piercing my cartridges with a second sterile needle while I injected the solution to give the air somewhere to escape. Your solution makes a lot of sense, though. Did you have any trouble drawing that much of a vacuum?
careful with the cartridges. the stopper can blow out if you try to fill one without giving the air somewhere to go.
 
I was planning on piercing my cartridges with a second sterile needle while I injected the solution to give the air somewhere to escape. Your solution makes a lot of sense, though. Did you have any trouble drawing that much of a vacuum?
No, no issue. Just pull the air out. I was using a vial this time though.

Just tried creating a vaccum in a cartridge and the stopper moved up to the top. Might not be able to create a vaccum, but if the stopper position is dependent on the volume it may still work.

I'll try doing an air purge into a cartridge later and report back.
 
Just tried creating a vaccum in a cartridge and the stopper moved up to the top.
That would really worry me. I don't have one in hand yet, but if the stopper moves forward, isn't the rear of the vial exposed to the atmosphere? That part's no longer going to be sterile, theoretically, and if you move the stopper backwards again then the non-sterile part becomes reconnected to the sterile inside.

Probably overthinking this, I see tons of people recommending buying cartridges from aliexpress which make no mention of being sterile in the first place and not reporting any problems.
 
Very good point @LEPacker. I'm actually embarrassed that didn't occur to me. And while it might be a slight risk, if nothing else I think it might defeat the purpose of filtering in the first place.

With that in mind, I'll stick with filtering into vials.
 
@fetefille, have you done any further tests on your Cobetter filters? You mentioned in your earlier post you thought there was a chance you did something wrong and your zero loss might be too good to be true.

I've now observed zero loss with the Cobetter filters after an air purge, but I'm also not 100% sure I did everything right. Just looking to compare notes.
I don’t think it’s zero, but dang near close. Still not satisfied though, lol. There’s no way for me to do it up right like Peptest, so my findings aren’t “real,” but for my satisfaction they work. I did notice a difference in the amount lost when I wet it vs when I didn’t. Wetting is important!
 
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Okay, quick update on my 2nd practice round. Still using the .65/.22 13mm Cobetter double filters.

Took my time and was super careful this round. Also tried an air purge. Filtered 1 ml of bac water. After the air purge, losses were neglectable. 2 units lost out of 100 filtered. Just to make sure I didn't damage the filter after the air purge I filtered a bit more water and there was no difference in the amount of resistance. So I don't think I damaged the filter with the air purge.

I'll continue to experiment and report back (and others please do the same), but based on this, it seems losses for the 13mm filters might be a bit overstated. At least for the Cobetter filters.

One important piece of advice, before doing an air purge, make sure the vial/cartridge you are filtering into has a vacuum. I created a vacuum in the vial I was using by removing air with an insilin syringe, pulling out 3ml of air. Trying to air purge without vaccum was how I lost .5ml of the 3ml filtered last time.
Hi, I'm about to try filtering for the first time tomorrow. Would you mind explaining the air purge? I keep seeing the term but I'm afraid I'm not quite grasping what it is/how to do it. I'm eager to avoid losing product or doing anything to introduce any contamination during this whole process.

Anyone else have any tips or tricks from doing this a few times? I'm going to go slowly but still feel bound to screw it up somehow.

I have sterile 1" luer lock needles, 3 ml syringes, Ks-tek 3 ml vials, exam gloves, and a stainless steel surface. I'm planning to reconstitute and filter T60s w/2.0 mL of Hospira bac water. Using peptidetest's .22 um PES filters.
 
What I did was draw up a bit of air behind the peptide in the syringe. Then push that air through the filter after pushing through the liquid.

Also flushed with bac water after. To make sure i didn't damage the filter, I made note of how much resistance there was before and after the air purge. If there was less resistance, then the filter was probably damaged, but the resistance was the same before/after (as best i could tell anyway, I don't have a way to measure).
 
Anyone else have any tips or tricks from doing this a few times?
My understanding is that after filtering the pep into the sterile vial, draw air from that vial while the needle is still inserted and push it back out again which should release remaining solution held in filter. So filtering and air purge is done via a single needle poke into the vial.
 
@PopTart, did you remove and re-attach the filter doing it this way? At least for the filters I have, it's very difficult to pull air in through the filter.

The way I did it was to draw air before drawing up the unfiltered peptide. But that was just based on what someone wrote on one of these boards, doesn't mean it's right. I think most everyone is learning as we go on this topic (including me if that wasn't obvious)
 
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@PopTart, did you remove and re-attach the filter doing it this way? At least for the filters I have, it's very difficult to pull air in through the filter.
To be honest, I haven’t filtered yet. Was hoping you would pop in here and help straighten out the process.

If I need to do that, should I separate the filter from the syringe while keeping the needle in the vial, draw air into syringe, reattach, then air purge?
 
There Would be pros and cons to doing it that way vs what I did. I had to keep more air in the syringe so less bac water. filling the syringe with the max 3ml my lure locks hold is another way to keep losses to a minimum.

Less bac water means less dilution, so higher losses in mg for the volume lost whuch is fixed.

But fewer steps with the way I did it.
 
If I need to do that, should I separate the filter from the syringe while keeping the needle in the vial, draw air into syringe, reattach, then air purge?
That sounds reasonable, but there may be something I'm overlooking. Like I said, I think we are all learning as we go on this.
 
Oh I hadn't even thought of less loss per more dilution. Hmmm.
 
No need to remove the needle from the syringe or from the vial as Chizi said :
1. Attach a needle to your syringe, draw your reconstituted solution.
2. Remove the needle and draw air in the syringe.
3. Attach a filter + another needle to the syringe and inject into a clean vial, syringe pointing upward (so the air is injected last).

Filter only goes one way, it you use the same to draw and inject it's not gonna be doing anything.

Look at the post in my signature of you want some more tricks.
 
The more I read, the more confused I become!

If I use this filter:
  • The end of the filter that connects to the syringe is a luer lock. Which means the syringe must be a luer lock.
  • The end of the filter that connects to the needle is a luer slip. Which means the needle must be a luer slip.
If I understand correctly, would that would mean I need two different types of needles:
  • a lock needle to connect to the syringe for drawing the reconstituted peptide
  • a slip needle to connect to the filter for filtering and injecting into a new vial?
Is that right?
 
The more I read, the more confused I become!

If I use this filter:
  • The end of the filter that connects to the syringe is a luer lock. Which means the syringe must be a luer lock.
  • The end of the filter that connects to the needle is a luer slip. Which means the needle must be a luer slip.
If I understand correctly, would that would mean I need two different types of needles:
  • a lock needle to connect to the syringe for drawing the reconstituted peptide
  • a slip needle to connect to the filter for filtering and injecting into a new vial?
Is that right?
You can slip a lock over the end of the filter
 
The more I read, the more confused I become!

If I use this filter:
  • The end of the filter that connects to the syringe is a luer lock. Which means the syringe must be a luer lock.
  • The end of the filter that connects to the needle is a luer slip. Which means the needle must be a luer slip.
If I understand correctly, would that would mean I need two different types of needles:
  • a lock needle to connect to the syringe for drawing the reconstituted peptide
  • a slip needle to connect to the filter for filtering and injecting into a new vial?
Is that right?
I don't use these exact filters, but I think any lure lock syringe will do.

Also, the filters you linked to are non- sterile per the website.
 

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