Syringe Filter
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Ok NOW I feel a little stupid...I was so busy looking for the double filter option on Amazon to match the Cobetter website choices, I missed the first bullet point under the pricing/quanity.
[Unique Structure]The double-layer asymmetric PES hydrophilic membranes behave graduated pore size structure, a larger size of prefilter and a 0.22μm sterilizing-grade final filter
scarywood75
Member
I've ordered some directly from Cobetter, we shall see how it goes.
bradyfan127
New_Member
Here's a link to some .22um 4mm filters. 200 pack for $100... Not sure if any of you are familiar with the brand or have previously tried.
filtrous.com
PTFE Single Layer (DX) Non-Sterile, Syringe Filter, 0.22um, 4mm Diameter, 200/unit
Filtrous' polytetrafluoroethylene membrane type filters are typically used for strong acid and alkali solvent filtration. They are great for degassing/clarifying aqueous samples because of the high temperature resistance.
filtrous.com
Chizikitide-1977
Registered
Also doesn't say if they're sterile.
I ordered the single layer from Cobetter (13mm, tiny!) for some sus cagri I have, they just came today. I used water, an empty vial, a luer lock syringe/needle, and one of the filters to practice with.
2ml water into the vial, pre-wet the filter, pulled the water out with syringe (made sure I drew it all out), removed needle, attached filter, reattached needle, pushed the water back into the vial.
There was resistance I didn’t expect (I also created a vacuum in the vial somehow, no clue), so it went slower than I expected. Removed the syringe part to do that “flush,” pulled air in, reattached, pushed through to flush the filter.
Finally, I pulled the contraption apart, and pulled up all of the water to see how much I’d lost. Virtually none. So little I’m going to have to do this a few more times to make sure.
Pic of the little filter
2ml water into the vial, pre-wet the filter, pulled the water out with syringe (made sure I drew it all out), removed needle, attached filter, reattached needle, pushed the water back into the vial.
There was resistance I didn’t expect (I also created a vacuum in the vial somehow, no clue), so it went slower than I expected. Removed the syringe part to do that “flush,” pulled air in, reattached, pushed through to flush the filter.
Finally, I pulled the contraption apart, and pulled up all of the water to see how much I’d lost. Virtually none. So little I’m going to have to do this a few more times to make sure.
Pic of the little filter
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Fonkymonky
New_Member
In the meantime I found the right filters (I hope 
) but apparently instead of the 13mm diameter I bought the 25mm diameter (see pictures) I hope this will not cause any problems? Can someone tell me the dimensions of the syringe and needle I have to get to match these? Also, is there a website appart from Amazon where I can find the sterile vials?
Thanks in advance!
) but apparently instead of the 13mm diameter I bought the 25mm diameter (see pictures) I hope this will not cause any problems? Can someone tell me the dimensions of the syringe and needle I have to get to match these? Also, is there a website appart from Amazon where I can find the sterile vials?Thanks in advance!
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moonpies4misfits
Research Enthusiast
Just make sure you get luer lock syringes. The needles will fit both luer lock and luer slip, 25g-27g are what I see a lot of people using
noteablequotable
Registered
Please let us know how this goes for you. Id definitely prefer this to standard insulin needles.
Fonkymonky
New_Member
Thanks!
noteablequotable
Registered
If you're worried about lost product the Peptide Test YouTube channel has a good video on this. Makes sense that you lost very little water.
I don't think that material is recommended for peptides. I think PES is what you want.Here's a link to some .22um 4mm filters. 200 pack for $100... Not sure if any of you are familiar with the brand or have previously tried.
PTFE Single Layer (DX) Non-Sterile, Syringe Filter, 0.22um, 4mm Diameter, 200/unit
Filtrous' polytetrafluoroethylene membrane type filters are typically used for strong acid and alkali solvent filtration. They are great for degassing/clarifying aqueous samples because of the high temperature resistance.
Not so much worried as surprised at how little I lost. Folks are really trying to hunt down the 4mm, but I think the 13mm appear to do just fine.
Fonkymonky
New_Member
I ordered the 25mm. Do you think it makes a big difference?
Someone please correct me if I'm wrong, but my understanding is that .25mm doesn't work well at all for the small volumes we're dealing with when reconstituting peptides. The amount that stays behind in the filter is something close to a ml.
It will, but still maybe not as much to make a difference to you, though I’m not you. Looking at the cobetter site, it says the “hold-up volume” is less than 25 microliters for the 13mm, less than 100 microliters for the 25mm.
So I think you’ll notice a little bit, but we’re talking microliters. How much does that little bit matter to you? And those numbers are pre-purge, I think. (Don’t quote me, I haven’t worked in a lab since 2012 and even then I wouldn’t have quoted me.)
and piggybacking off of the last person, the site says the 25mm are best for samples 10ml to 100ml, so idk
I’m looking at couple other sites and they say their 25mm can be used for as little as 2ml, with a hold up volume of less than 50 to as much as 115 microliters, so maybe it’s brand specific?
bradyfan127
New_Member
Thanks for letting me know. I've been going back and forth on this. Chatting on another board and reading information on here has actually helped. I've only been here one day and the conversation is WAY WAY more productive.
