I was planning on piercing my cartridges with a second sterile needle while I injected the solution to give the air somewhere to escape. Your solution makes a lot of sense, though. Did you have any trouble drawing that much of a vacuum?
Syringe Filter
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That is what I do. It releases the vacuum and equalizes the pressure while you fill it.
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careful with the cartridges. the stopper can blow out if you try to fill one without giving the air somewhere to go.
Chizikitide-1977
Registered
No, no issue. Just pull the air out. I was using a vial this time though.
Just tried creating a vaccum in a cartridge and the stopper moved up to the top. Might not be able to create a vaccum, but if the stopper position is dependent on the volume it may still work.
I'll try doing an air purge into a cartridge later and report back.
That would really worry me. I don't have one in hand yet, but if the stopper moves forward, isn't the rear of the vial exposed to the atmosphere? That part's no longer going to be sterile, theoretically, and if you move the stopper backwards again then the non-sterile part becomes reconnected to the sterile inside.
Probably overthinking this, I see tons of people recommending buying cartridges from aliexpress which make no mention of being sterile in the first place and not reporting any problems.
Chizikitide-1977
Registered
I don’t think it’s zero, but dang near close. Still not satisfied though, lol. There’s no way for me to do it up right like Peptest, so my findings aren’t “real,” but for my satisfaction they work. I did notice a difference in the amount lost when I wet it vs when I didn’t. Wetting is important!
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Hi, I'm about to try filtering for the first time tomorrow. Would you mind explaining the air purge? I keep seeing the term but I'm afraid I'm not quite grasping what it is/how to do it. I'm eager to avoid losing product or doing anything to introduce any contamination during this whole process.
Anyone else have any tips or tricks from doing this a few times? I'm going to go slowly but still feel bound to screw it up somehow.
I have sterile 1" luer lock needles, 3 ml syringes, Ks-tek 3 ml vials, exam gloves, and a stainless steel surface. I'm planning to reconstitute and filter T60s w/2.0 mL of Hospira bac water. Using peptidetest's .22 um PES filters.
Chizikitide-1977
Registered
What I did was draw up a bit of air behind the peptide in the syringe. Then push that air through the filter after pushing through the liquid.
Also flushed with bac water after. To make sure i didn't damage the filter, I made note of how much resistance there was before and after the air purge. If there was less resistance, then the filter was probably damaged, but the resistance was the same before/after (as best i could tell anyway, I don't have a way to measure).
Also flushed with bac water after. To make sure i didn't damage the filter, I made note of how much resistance there was before and after the air purge. If there was less resistance, then the filter was probably damaged, but the resistance was the same before/after (as best i could tell anyway, I don't have a way to measure).
My understanding is that after filtering the pep into the sterile vial, draw air from that vial while the needle is still inserted and push it back out again which should release remaining solution held in filter. So filtering and air purge is done via a single needle poke into the vial.
Chizikitide-1977
Registered
@PopTart, did you remove and re-attach the filter doing it this way? At least for the filters I have, it's very difficult to pull air in through the filter.
The way I did it was to draw air before drawing up the unfiltered peptide. But that was just based on what someone wrote on one of these boards, doesn't mean it's right. I think most everyone is learning as we go on this topic (including me if that wasn't obvious)
The way I did it was to draw air before drawing up the unfiltered peptide. But that was just based on what someone wrote on one of these boards, doesn't mean it's right. I think most everyone is learning as we go on this topic (including me if that wasn't obvious)
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To be honest, I haven’t filtered yet. Was hoping you would pop in here and help straighten out the process.
If I need to do that, should I separate the filter from the syringe while keeping the needle in the vial, draw air into syringe, reattach, then air purge?
Chizikitide-1977
Registered
There Would be pros and cons to doing it that way vs what I did. I had to keep more air in the syringe so less bac water. filling the syringe with the max 3ml my lure locks hold is another way to keep losses to a minimum.
Less bac water means less dilution, so higher losses in mg for the volume lost whuch is fixed.
But fewer steps with the way I did it.
Less bac water means less dilution, so higher losses in mg for the volume lost whuch is fixed.
But fewer steps with the way I did it.
Chizikitide-1977
Registered
That sounds reasonable, but there may be something I'm overlooking. Like I said, I think we are all learning as we go on this.
scarywood75
Member
No need to remove the needle from the syringe or from the vial as Chizi said :
1. Attach a needle to your syringe, draw your reconstituted solution.
2. Remove the needle and draw air in the syringe.
3. Attach a filter + another needle to the syringe and inject into a clean vial, syringe pointing upward (so the air is injected last).
Filter only goes one way, it you use the same to draw and inject it's not gonna be doing anything.
Look at the post in my signature of you want some more tricks.
1. Attach a needle to your syringe, draw your reconstituted solution.
2. Remove the needle and draw air in the syringe.
3. Attach a filter + another needle to the syringe and inject into a clean vial, syringe pointing upward (so the air is injected last).
Filter only goes one way, it you use the same to draw and inject it's not gonna be doing anything.
Look at the post in my signature of you want some more tricks.
The more I read, the more confused I become!
If I use this filter:
If I use this filter:
- The end of the filter that connects to the syringe is a luer lock. Which means the syringe must be a luer lock.
- The end of the filter that connects to the needle is a luer slip. Which means the needle must be a luer slip.
- a lock needle to connect to the syringe for drawing the reconstituted peptide
- a slip needle to connect to the filter for filtering and injecting into a new vial?
You can slip a lock over the end of the filter
Chizikitide-1977
Registered
I don't use these exact filters, but I think any lure lock syringe will do.
Also, the filters you linked to are non- sterile per the website.
